抗载脂蛋白AI单克隆抗体：制备与特性分析

Monoclonal antibodies to apolipoprotein AI: generation and characterization.

作者信息

Falero G, Rodriguez I, Rojas A

机构信息

Hybridomas and Monoclonal Antibodies Laboratory, National Center for Scientific Research, Havana.

出版信息

Folia Biol (Praha). 1993;39(1):14-22.

PMID:7688698

Abstract

BALB/c mice were immunized with apolipoprotein (apo AI)--high density lipoprotein (HDL) conjugate. By polyethylene glycol-induced fusion of isolated spleen cells with the myeloma cell line P3 X63 Ag8 6.5.3, three different hybridomas were obtained and characterized. Two of them were found to secrete antibodies of the IgG2a subclass, whereas the third produced antibodies of the IgG1 type. Binding capacities to 125I-apo AI and 125I-HDL were higher than 90% in all cases. The isolated antibodies recognize independent epitopes on the apo AI molecule and bind isolated HDL and serum-HDL with different affinities.

摘要

用载脂蛋白（apo AI）-高密度脂蛋白（HDL）偶联物免疫BALB/c小鼠。通过聚乙二醇诱导分离的脾细胞与骨髓瘤细胞系P3 X63 Ag8 6.5.3融合，获得并鉴定了三种不同的杂交瘤。发现其中两种分泌IgG2a亚类抗体，而第三种产生IgG1型抗体。在所有情况下，对125I-apo AI和125I-HDL的结合能力均高于90%。分离出的抗体识别apo AI分子上的独立表位，并以不同亲和力结合分离的HDL和血清HDL。