Hagel L, Hartmann A, Lund K
R&D Department, Pharmacia LKB Biotechnology AB, Uppsala, Sweden.
J Chromatogr. 1993 Jul 2;641(1):63-70. doi: 10.1016/0021-9673(93)83459-6.
Analytical gel filtration was used for the study of molecular size distribution of clinical dextran in serum and urine for the purpose of evaluations of changes in the human glomerular barrier function. The column was calibrated in terms of solute size using a simple and accurate technique recently described. Only one sample of a dextran possessing a broad molecular mass distribution was necessary for the calibration procedure and the calculations were performed using an ordinary spreadsheet. The accuracy of the calibration, as evaluated by protein samples, is better than 95%. The simplicity makes the method suitable for use in laboratories not normally specializing in analytical gel filtration. Calibration in terms of size is preferably done with respect to viscosity radius to obtain relevant information about the permeability of dextran into porous membranes.
为评估人类肾小球屏障功能的变化，采用分析凝胶过滤法研究临床右旋糖酐在血清和尿液中的分子大小分布。使用最近描述的一种简单而准确的技术，根据溶质大小对柱子进行校准。校准过程只需一个具有广泛分子量分布的右旋糖酐样品，并且计算使用普通电子表格进行。通过蛋白质样品评估，校准的准确性优于95%。该方法的简单性使其适用于通常不专门从事分析凝胶过滤的实验室。最好根据粘度半径进行大小校准，以获得有关右旋糖酐进入多孔膜渗透性的相关信息。
