Smith Q T, Camp S J
Department of Oral Science, University of Minnesota, Minneapolis 55455.
J Clin Periodontol. 1993 Jul;20(6):418-24. doi: 10.1111/j.1600-051x.1993.tb00382.x.
Extrasulcular substances such as saliva, supragingival plaque and salivary sediment may be contaminants in gingival crevicular fluid (GCF) collected with Periopaper. This report provides data obtained with salivary amylase as a marker for these substances in GCF. Amylase was a common constituent of GCF collected from sites with clinical health and with clinical signs of periodontitis. Rinsing the mouth with water reduced, but did not eliminate amylase in GCF. More frequent (p < 0.01) and greater (p < 0.001) contamination of GCF with amylase occurred in samples from periodontitis than from healthy subjects. The volume of saliva required to give the amylase in the GCF was calculated. This volume exceeded the GCF volume in 21% of samples collected without a water rinse. Thus, oral constituents other than saliva likely contribute to GCF amylase. Small quantities of plaque and salivary sediment (9.6 +/- 5.9, 3.4 +/- 2.0 micrograms protein) provided amylase from a saliva volume equal to the GCF volume in health (0.23 microliters). The above and other data presented here show that extrasulcular substances likely are frequent constituents of GCF collected with Periopaper. Reporting GCF constituents as quantities/sample appears least subject to error from the contamination by extrasulcular substances.
诸如唾液、龈上菌斑和唾液沉积物等龈沟外物质可能是用牙周试纸采集的龈沟液（GCF）中的污染物。本报告提供了以唾液淀粉酶作为这些物质在GCF中的标志物所获得的数据。淀粉酶是从临床健康部位和有牙周炎临床体征部位采集的GCF的常见成分。用水漱口可减少但不能消除GCF中的淀粉酶。与健康受试者相比，牙周炎患者样本中GCF被淀粉酶污染的频率更高（p < 0.01）且污染程度更大（p < 0.001）。计算了使GCF中出现淀粉酶所需的唾液量。在未用水冲洗采集的样本中，该量超过GCF量的情况占21%。因此，除唾液外的口腔成分可能也对GCF淀粉酶有贡献。少量菌斑和唾液沉积物（蛋白质分别为9.6±5.9、3.4±2.0微克）在健康状态下从与GCF量相等的唾液量（0.23微升）中提供淀粉酶。此处呈现的上述及其他数据表明，龈沟外物质可能是用牙周试纸采集的GCF的常见成分。将GCF成分报告为每样本的量似乎受龈沟外物质污染误差影响最小。
