射频爆发型电磁场辐射使大鼠小脑一氧化氮和环磷酸鸟苷增加。

Increase in nitric oxide and cyclic GMP of rat cerebellum by radio frequency burst-type electromagnetic field radiation.

作者信息

Miura M, Takayama K, Okada J

机构信息

Department of Physiology First Division, Gunma University School of Medicine, Maebashi-shi, Japan.

出版信息

J Physiol. 1993 Feb;461:513-24. doi: 10.1113/jphysiol.1993.sp019526.

DOI:10.1113/jphysiol.1993.sp019526

PMID:7688808

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1175270/

Abstract

Using rat cerebellum supernatant, the effects of radio frequency (RF) burst-type electromagnetic (EM) field radiation on the production of cyclic GMP were examined under various conditions. The radiation was generated by a generator coil, and set at a 10 MHz radiation frequency, a 50% burst time, a 10 kHz burst rate and a 5 V peak-to-peak generator voltage. 2. When the cerebellum supernatant was incubated with both exogenous L-arginine (nitric oxide (NO) donor) and NADPH, and irradiated by an RF burst-type EM field, the production of cyclic GMP was increased significantly from a level of 21-22 nmol min-1 (g tissue)-1 to 25-26 nmol min-1 (g tissue)-1. By contrast, such an effect was not found when the cerebellum supernatant was irradiated by an RF volley-type EM field. 3. When neither L-arginine nor NADPH were added to the cerebellum supernatant, the production of cyclic GMP was lowered to a level of 6 nmol min-1 (g tissue)-1 and the radiation effect was not found. When the cerebellum supernatant was chelated with EDTA, the production of cyclic GMP was lowered to a level of 7 nmol min-1 (g tissue)-1 and the radiation effect was not found. 4. Incubation with Methylene Blue, a guanylate cyclase inhibitor, lowered the production of cyclic GMP to a level of 10-12 nmol min-1 (g tissue)-1, and the radiation effect did not occur. On incubation with a NO synthase inhibitor, either NG-methyl-L-arginine or N omega-nitro-L-arginine methyl ester, the production of cyclic GMP was lowered to a level of 10-12 nmol min-1 (g tissue)-1 or 5-9 nmol min-1 (g tissue)-1 respectively, and the radiation effect was not observed. 5. Using electrochemical NO probes, the production of NO in the cerebellum supernatant was detected. The concentration of NO increased gradually after the onset of the EM field radiation. The radiation effect persisted, and reached a maximum after the cessation of the radiation. 6. In an in vivo study, the arterioles of the frog web were dilated by the radiation, and this radiation effect was almost completely abolished by the addition of a NO synthase inhibitor. This indicates that radiation activates NO synthase and ultimately induces vasodilatation.

摘要

使用大鼠小脑上清液，在各种条件下研究了射频（RF）猝发型电磁场辐射对环鸟苷酸（cGMP）产生的影响。辐射由发生器线圈产生，设置辐射频率为10 MHz、猝发时间为50%、猝发速率为10 kHz以及发生器峰 - 峰值电压为5 V。2. 当小脑上清液与外源性L - 精氨酸（一氧化氮（NO）供体）和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）一起孵育，并受到RF猝发型电磁场辐射时，cGMP的产生从21 - 22 nmol·min⁻¹·（g组织）⁻¹显著增加到25 - 26 nmol·min⁻¹·（g组织）⁻¹。相比之下，当小脑上清液受到RF齐射型电磁场辐射时未发现这种效应。3. 当小脑上清液中既不添加L - 精氨酸也不添加NADPH时，cGMP的产生降至6 nmol·min⁻¹·（g组织）⁻¹的水平，且未发现辐射效应。当小脑上清液用乙二胺四乙酸（EDTA）螯合时，cGMP的产生降至7 nmol·min⁻¹·（g组织）⁻¹的水平，且未发现辐射效应。4. 用鸟苷酸环化酶抑制剂亚甲蓝孵育后，cGMP的产生降至10 - 12 nmol·min⁻¹·（g组织）⁻¹的水平，且未发生辐射效应。用NO合酶抑制剂，即NG - 甲基 - L - 精氨酸或Nω - 硝基 - L - 精氨酸甲酯孵育后，cGMP的产生分别降至10 - 12 nmol·min⁻¹·（g组织）⁻¹或5 - 9 nmol·min⁻¹·（g组织）⁻¹的水平，且未观察到辐射效应。5. 使用电化学NO探针，检测了小脑上清液中NO的产生。电磁场辐射开始后，NO浓度逐渐增加。辐射效应持续存在，并在辐射停止后达到最大值。6. 在一项体内研究中，青蛙蹼的小动脉因辐射而扩张，并且添加NO合酶抑制剂后这种辐射效应几乎完全消除。这表明辐射激活了NO合酶并最终诱导血管舒张。