Katzenellenbogen E, Gamian A, Romanowska E, Dabrowski U, Dabrowski J
Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1058-64. doi: 10.1006/bbrc.1993.1929.
The 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of Hafnia alvei strains 2 and 1211 using gel filtration. For purification the trisaccharide fraction was rechromatographed on a Bio-Gel P2 column. On the basis of sugar and methylation analyses and 1H NMR spectroscopy the trisaccharide was identified as 3-deoxy-7-O-(6-O-acetyl-alpha-D-galactopyranosyl)-4-O-(alpha-L-glycero-D - mannoheptopyranosyl)-D-manno-2-octulopyranosic acid. The occurrence of such bifurcate Kdo-containing structure has not been previously reported in Enterobacteriaceae.
通过凝胶过滤从蜂房哈夫尼亚菌菌株2和1211脂多糖酸水解后得到的碳水化合物材料中分离出含3-脱氧辛糖酸的三糖。为了纯化，将三糖级分在Bio-Gel P2柱上重新进行色谱分离。基于糖分析、甲基化分析和1H核磁共振光谱，该三糖被鉴定为3-脱氧-7-O-(6-O-乙酰基-α-D-吡喃半乳糖基)-4-O-(α-L-甘油-D-甘露庚吡喃糖基)-D-甘露-2-辛吡喃糖酸。此前在肠杆菌科中尚未报道过这种含分叉Kdo的结构的存在。
