酿酒酵母和卡尔斯伯酵母线粒体DNA精细结构的比较：部分变性分子的电子显微镜观察

Christiansen G, Christiansen C

Nucleic Acids Res. 1976 Feb;3(2):465-76. doi: 10.1093/nar/3.2.465.

Denaturation-maps of mitochondrial DNA from Saccharomyces cerevisiae and S. carlsbergensis have been derived from electron microscopic observations of partially denatured complete circular molecules and large fragments of these circles. The mitochondrial DNA from the two species differ by 6% in total length, but seems from the maps to contain some regions of apparent close homology. The cleavage pattern of the two DNAs by the restriction endonuclease EcoRI is compared by gel electrophoresis.

酿酒酵母和卡尔斯伯酵母线粒体DNA的变性图谱是通过对部分变性的完整环状分子及其大的片段进行电子显微镜观察得到的。这两个物种的线粒体DNA全长相差6%，但从图谱来看似乎包含一些明显高度同源的区域。通过凝胶电泳比较了两种DNA经限制性内切酶EcoRI的切割模式。