Zhang Y Q, Su H C, Huang Y Q
Department of Histology and Embryology, Fourth Military Medical University, Xi'an.
Shi Yan Sheng Wu Xue Bao. 1993 Mar;26(1):65-71.
CD57, a glycoprotein, is a specific antigen presented on the surface of human natural killer cells and killer cells. The monoclonal antibody to CD57 has been available. In this study, the avidin-biotin-peroxidase complex (ABC) technique visualized with glucose oxidase-DAB-nickle (GDN) was used to detect the expression of CD57 in endocrine cells of human pancreas. The results showed that the CD57 immunoreactive (CD57-IR) cells were scattered throughout human pancreas. Most of positive cells were localized in the pancreatic islets. Grouped or single cells occurred intercalated between acinar cells, as well as in the epithelium of pancreatic ducts. The anti-CD57-monoclonal antibody stained the myelin sheaths of nerve fibers in all tissues investigated. Alternative sections immunostained with antibodies against bioactive peptides proved the coexistence of CD57 and Glu, SS, HPP or PS in endocrine cells. The significance of the expression of CD57 immunoreactivity in endocrine cells of human pancreas was discussed.
CD57是一种糖蛋白，是人类自然杀伤细胞和杀伤细胞表面呈现的一种特异性抗原。针对CD57的单克隆抗体已经可以获得。在本研究中，采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）技术并用葡萄糖氧化酶-DAB-镍（GDN）显色，以检测人胰腺内分泌细胞中CD57的表达。结果显示，CD57免疫反应性（CD57-IR）细胞散在于人胰腺各处。大多数阳性细胞位于胰岛。成群或单个的细胞插在腺泡细胞之间，以及胰腺导管上皮中。抗CD57单克隆抗体使所有研究组织中的神经纤维髓鞘着色。用针对生物活性肽的抗体进行免疫染色的连续切片证明，CD57与内分泌细胞中的谷氨酸（Glu）、生长抑素（SS）、人胰多肽（HPP）或胰泌素（PS）共存。讨论了人胰腺内分泌细胞中CD57免疫反应性表达的意义。
