Bouzahzah F, Heinen E, Antoine N, Bosseloir A, Mancini I, Simar L J
Institute of Human Histology, University of Liège, Belgium.
Eur J Morphol. 1993 Mar-Jun;31(1-2):82-6.
The presence of CD4+, CD57+ T cells in the germinal centers has been reported by several authors. The CD57 antigen is also expressed by natural killer (NK) cells. We purified CD57+ cells from human tonsils and blood by microdissection, rosetting with sheep red blood cells and magnetic cell sorting (MACS) and examined the ultrastructural morphology of these cells. Clear differences were found in cell aspect: blood NK contained large granules which were not found in the tonsillar CD57+ cells. These latter appeared medium-sized and not fully activated. After immunolabeling, the tonsillar CD57+ cells were mainly found in the light zone of the germinal centers.
几位作者报告了生发中心中存在CD4 +、CD57 + T细胞。CD57抗原也由自然杀伤（NK）细胞表达。我们通过显微切割、与绵羊红细胞形成玫瑰花结以及磁性细胞分选（MACS）从人扁桃体和血液中纯化了CD57 +细胞，并检查了这些细胞的超微结构形态。在细胞外观上发现了明显差异：血液NK细胞含有大颗粒，而扁桃体CD57 +细胞中未发现这些大颗粒。后者看起来中等大小且未完全活化。免疫标记后，扁桃体CD57 +细胞主要存在于生发中心的明区。
