牙鲆（Paralichthys olivaceus）干扰素cDNA的克隆与表达

Cloning and expression of flatfish (Paralichthys olivaceus) interferon cDNA.

作者信息

Tamai T, Shirahata S, Noguchi T, Sato N, Kimura S, Murakami H

机构信息

Taiyo Central R&D Institute, Taiyo Fishery Co. Ltd., Ibaraki, Japan.

出版信息

Biochim Biophys Acta. 1993 Aug 19;1174(2):182-6. doi: 10.1016/0167-4781(93)90112-q.

DOI:10.1016/0167-4781(93)90112-q

PMID:7689338

Abstract

Fish interferon (IFN) cDNA was first cloned from the cDNA library of immortalized flatfish leukocytes. The clone contains an open reading frame that encodes a 138 amino acid polypeptide including a glycosylation site and a signal peptide containing 30 amino acids. BHK-21 cells transfected with the INF-expression plasmid produced active recombinant IFN (about 16 kDa) which was then purified by WGA agarose affinity chromatography. This recombinant IFN inhibited infection of fish cells with the Hirame (flatfish) rhabdovirus.

摘要

鱼类干扰素（IFN）cDNA最初是从永生化比目鱼白细胞的cDNA文库中克隆得到的。该克隆包含一个开放阅读框，其编码一个138个氨基酸的多肽，该多肽包括一个糖基化位点和一个含有30个氨基酸的信号肽。用INF表达质粒转染的BHK-21细胞产生了活性重组干扰素（约16 kDa），然后通过WGA琼脂糖亲和层析进行纯化。这种重组干扰素抑制了比目鱼弹状病毒对鱼类细胞的感染。