Sheldon A, Flego L, Zola H
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia.
J Leukoc Biol. 1993 Aug;54(2):161-7. doi: 10.1002/jlb.54.2.161.
By using high-sensitivity fluorescence and flow cytometry, it is possible to show that 30-40% of lymphocytes from PBL express the p55 chain of the IL-2 receptor, whereas the p75 chain is expressed at low concentrations on most lymphocytes without in vitro activation. The availability of a second fluorochrome capable of high sensitivity allows simultaneous analysis of p55 and p75, albeit with some sacrifice in sensitivity. Two-color analysis shows that a small proportion of cells (1-6%) coexpress measurable concentrations of both chains of the IL-2 receptor, and three-color studies show that these cells are predominantly CD4-positive T cells and express the CD45R0 isoform of the leucocyte-common antigen, i.e., have the phenotype of activated helper T cells. These cells may be a useful indicator of immune activation.
通过使用高灵敏度荧光和流式细胞术，可以显示来自外周血淋巴细胞（PBL）的30%-40%的淋巴细胞表达白细胞介素-2受体的p55链，而p75链在大多数未经体外激活的淋巴细胞上以低浓度表达。能够实现高灵敏度的第二种荧光染料的可用性允许同时分析p55和p75，尽管在灵敏度上有所牺牲。双色分析显示一小部分细胞（1%-6%）共表达可测量浓度的白细胞介素-2受体的两条链，三色研究显示这些细胞主要是CD4阳性T细胞，并表达白细胞共同抗原的CD45R0同种型，即具有活化辅助性T细胞的表型。这些细胞可能是免疫激活的有用指标。
