Stocks S C, Kerr M A
Department of Pathology, University of Dundee, Ninewells Hospital, U.K.
Biochem Biophys Res Commun. 1993 Aug 31;195(1):478-83. doi: 10.1006/bbrc.1993.2068.
The neutrophil surface carbohydrate groups LewisX and sialyl LewisX have previously been shown to interact with the vascular selectins E- and P-selectin. However, the proteins expressing these carbohydrate groups have not been fully characterised. We show that these carbohydrate groups, and the structurally related Lewisa group, are all carried predominantly on a sub-population of the carcinoembryonic antigen (CEA) related NCA-160, which is also recognised by CD66 antibodies. We demonstrate that the related NCA-160, NCA-90/95 and CD67 all undergo an increase in surface expression in response to fMLP stimulation, and that this increase is greater than that observed for the sialyl and non-sialyl LewisX carbohydrate groups. These data suggest that the expression of differently glycosylated forms of NCA is independently regulated.
中性粒细胞表面碳水化合物基团LewisX和唾液酸化LewisX此前已被证明可与血管选择素E-选择素和P-选择素相互作用。然而，表达这些碳水化合物基团的蛋白质尚未得到充分表征。我们发现，这些碳水化合物基团以及结构相关的Lewis a基团主要携带在癌胚抗原（CEA）相关的NCA-160亚群上，CD66抗体也可识别该亚群。我们证明，相关的NCA-160、NCA-90/95和CD67在受到fMLP刺激后表面表达均增加，且这种增加大于唾液酸化和非唾液酸化LewisX碳水化合物基团的增加。这些数据表明，NCA不同糖基化形式的表达是独立调节的。
