Doutrelant O, Poulain P, Carette B
INSERM U 156, Lille, France.
Brain Res. 1993 Jul 2;615(2):335-41. doi: 10.1016/0006-8993(93)90046-p.
The distribution both of Met-enkephalinergic nerve terminals and of calbindin-containing neurons was investigated in the guinea-pig lateral septum, using a double-immunostaining technique. The findings show that the two immunoreactivities overlapped for neurons located in areas of the dorsal part and of the mediolateral part of the lateral septum. Nine cells of the mediolateral part which were electrophysiologically characterized and intracellularly labelled were subjected to the double-immunostaining protocol. All these cells displayed characteristic discharges due to the activation of high-threshold Ca2+ conductances. Two of them contained calbindin and were the target of enkephalinergic inputs; they possessed somatic spines. These data demonstrate: (1) that the guinea-pig lateral septum contains subpopulations of calbindin neurons which are postsynaptic to enkephalinergic inputs; (2) that Ca2+ conductances are not related to the presence of calbindin; (3) that somatospiny neurons, which are involved in the regulation of the hippocampo-septo-hypothalamic complex, contain calbindin and are the target of enkephalinergic endings.
运用双重免疫染色技术，对豚鼠外侧隔中脑啡肽能神经终末和含钙结合蛋白的神经元的分布进行了研究。研究结果表明，对于位于外侧隔背侧部分和中外侧部分区域的神经元，这两种免疫反应性相互重叠。对9个经电生理学特征鉴定并进行细胞内标记的中外侧部分的细胞进行了双重免疫染色实验。所有这些细胞由于高阈值Ca2+电导的激活而呈现出特征性放电。其中两个细胞含有钙结合蛋白，是脑啡肽能输入的靶点；它们具有体细胞棘。这些数据表明：（1）豚鼠外侧隔含有钙结合蛋白神经元亚群，这些神经元是脑啡肽能输入的突触后神经元；（2）Ca2+电导与钙结合蛋白的存在无关；（3）参与海马-隔-下丘脑复合体调节的体棘神经元含有钙结合蛋白，并且是脑啡肽能终末的靶点。
