Pottosin I I
Laboratory of Membrane Biophysics, Russian Academy of Sciences, Pushchino Moscow Region.
FEBS Lett. 1993 Sep 13;330(2):211-4. doi: 10.1016/0014-5793(93)80275-y.
The voltage dependence of large conductance channels in the intact chloroplast envelope of Nitellopsis obtusa was examined using the patch-clamp technique. The channel switched to the lower conducting substates with amplitudes of around 45 and 20% of that in the open state when potentials larger than 30 mV were applied. The steepness of the voltage dependence approximately corresponds to 4 elementary charges being transferred across the entire voltage drop to close the channel both at positive and negative potentials. The transition to the closed substates could also be induced by König's polyanion, a well known modulator of the mitochondrial outer membrane channel.
使用膜片钳技术研究了钝节拟丽藻完整叶绿体被膜中大通量通道的电压依赖性。当施加大于30 mV的电位时,通道切换到较低导通亚态,其幅度约为开放态的45%和20%。电压依赖性的陡峭程度大致相当于在正电位和负电位下,有4个基本电荷穿过整个电压降以关闭通道。向关闭亚态的转变也可由线粒体外膜通道的著名调节剂柯尼格多聚阴离子诱导。