Hatori N, Haga Y, Sjöquist P O, Nordlander M, Rydén L
Department of Cardiology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
J Cardiovasc Pharmacol. 1993 Jul;22(1):160-6. doi: 10.1097/00005344-199307000-00024.
The effect of the vasoselective calcium antagonist felodipine on myocardial necrosis was studied in 14 anesthetized pigs subjected to 45-min occlusion of the left anterior descending coronary artery (LAD) followed by 24-h reperfusion. Felodipine (7 nmol/kg, n = 7), or the corresponding amount of vehicle diluted in 300 ml saline (n = 7) was infused in the great cardiac vein for 30 min beginning 5 min before onset of reperfusion. Regional myocardial function was measured as percentage of systolic segment shortening (%SS) by sonomicrometry. The recovery in ischemic myocardium was significantly already better among felodipine-treated animals after 30-min reperfusion (mean +/- SD = 9.1 +/- 6.1 vs. 0.1 +/- 3.2%, p < 0.05). The improved recovery in the felodipine group persisted throughout the observation period; %SS was 8.5 +/- 5.9% after 24-h reperfusion. No significant improvement was observed in the vehicle group after 24 h (%SS = 2.7 +/- 2.0%, p < 0.05). The area of the infarct was measured by triphenyl tetrazolium chloride staining. When expressed as percentage of the left ventricle, the infarct was smaller in the felodipine group (8.2 +/- 4.0%) than in the vehicle group (14.6 +/- 3.5%; p < 0.01). The corresponding values for infarct size expressed as percentage of the area at risk was 38.6 +/- 18.9% in the felodipine group and 69.8 +/- 6.7% in the vehicle group (p < 0.01). Peak plasma felodipine concentration was reached 30 min after onset of reperfusion (4.9 +/- 1.2 nM).(ABSTRACT TRUNCATED AT 250 WORDS)
在14只麻醉猪身上研究了血管选择性钙拮抗剂非洛地平对心肌坏死的影响。这些猪接受左冠状动脉前降支（LAD）45分钟闭塞，随后再灌注24小时。在再灌注开始前5分钟，于冠状大静脉内输注非洛地平（7 nmol/kg，n = 7）或等量溶解于300 ml生理盐水中的溶媒（n = 7），持续30分钟。采用超声心动图测量局部心肌功能，以收缩期节段缩短百分比（%SS）表示。再灌注30分钟后，非洛地平治疗组缺血心肌的恢复情况已明显更好（均值±标准差= 9.1±6.1对0.1±3.2%，p < 0.05）。非洛地平组的恢复改善在整个观察期持续存在；再灌注24小时后%SS为8.5±5.9%。溶媒组24小时后未观察到明显改善（%SS = 2.7±2.0%，p < 0.05）。通过氯化三苯基四氮唑染色测量梗死面积。以左心室百分比表示时，非洛地平组梗死面积（8.2±4.0%）小于溶媒组（14.6±3.5%；p < 0.01）。以危险区域面积百分比表示的梗死大小，非洛地平组为38.6±18.9%，溶媒组为69.8±6.7%（p < 0.01）。再灌注开始后30分钟达到血浆非洛地平峰值浓度（4.9±1.2 nM）。（摘要截短于250字）
