• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

1型人类免疫缺陷病毒进入表达CD4的人类细胞系所需的辅助因子

Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.

作者信息

Harrington R D, Geballe A P

机构信息

Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.

出版信息

J Virol. 1993 Oct;67(10):5939-47. doi: 10.1128/JVI.67.10.5939-5947.1993.

DOI:10.1128/JVI.67.10.5939-5947.1993
PMID:7690415
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238014/
Abstract

Expression of the human immunodeficiency virus type 1 (HIV-1) receptor CD4 on many nonhuman and some human cell lines is not sufficient to permit HIV-1 infection. We describe a human glioblastoma cell line (U373-MG) which remains resistant to HIV-1 despite the added expression of an authentic CD4 molecule. The block to HIV-1 infection of these cells is strain independent and appears to be at viral entry. Heterokaryons of CD4-expressing U373-MG (U373-CD4) cells fused to HeLa cells allow HIV-1 entry. A U373-CD4/HeLa hybrid clone allows efficient HIV-1 replication. These results suggest that HeLa cells express a factor(s) that can complement the viral entry defect of U373-CD4 cells and is necessary for efficient CD4-mediated HIV-1 infection.

摘要

人类免疫缺陷病毒1型（HIV-1）受体CD4在许多非人类和一些人类细胞系中的表达不足以使HIV-1感染。我们描述了一种人胶质母细胞瘤细胞系（U373-MG），尽管添加了真实的CD4分子表达，但该细胞系对HIV-1仍具有抗性。这些细胞对HIV-1感染的阻断是不依赖毒株的，并且似乎发生在病毒进入阶段。将表达CD4的U373-MG（U373-CD4）细胞与HeLa细胞融合形成的异核体允许HIV-1进入。一个U373-CD4/HeLa杂交克隆允许HIV-1高效复制。这些结果表明，HeLa细胞表达一种因子，该因子可以弥补U373-CD4细胞的病毒进入缺陷，并且是CD4介导的HIV-1有效感染所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/5aea5b845e36/jvirol00031-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/0ab328678ba3/jvirol00031-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/c0359267414e/jvirol00031-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/ad57c15be427/jvirol00031-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/5aea5b845e36/jvirol00031-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/0ab328678ba3/jvirol00031-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/c0359267414e/jvirol00031-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/ad57c15be427/jvirol00031-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f438/238014/5aea5b845e36/jvirol00031-0246-a.jpg

相似文献

1
Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.1型人类免疫缺陷病毒进入表达CD4的人类细胞系所需的辅助因子
J Virol. 1993 Oct;67(10):5939-47. doi: 10.1128/JVI.67.10.5939-5947.1993.
2
Human immunodeficiency virus type-1 susceptible whole cell and microcell hybrids.1型人类免疫缺陷病毒易感全细胞和微细胞杂种
Ann Clin Lab Sci. 1996 Nov-Dec;26(6):522-30.
3
Factors involved in entry of the human immunodeficiency virus type 1 into permissive cells: lack of evidence of a role for CD26.人类免疫缺陷病毒1型进入易感细胞所涉及的因素：缺乏CD26起作用的证据
J Virol. 1994 Oct;68(10):6535-46. doi: 10.1128/JVI.68.10.6535-6546.1994.
4
A rodent cell line permissive for entry and reverse transcription of human immunodeficiency virus type 1 has a pre-integration block to productive infection.
J Gen Virol. 1994 Oct;75 ( Pt 10):2615-23. doi: 10.1099/0022-1317-75-10-2615.
5
A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus type 1 strains.一种参与嗜T细胞的1型人类免疫缺陷病毒株融合与进入的七跨膜结构域受体。
J Virol. 1996 Sep;70(9):6288-95. doi: 10.1128/JVI.70.9.6288-6295.1996.
6
CD4 down-modulation during infection of human T cells with human immunodeficiency virus type 1 involves independent activities of vpu, env, and nef.人类免疫缺陷病毒1型感染人类T细胞期间的CD4下调涉及vpu、env和nef的独立活性。
J Virol. 1996 Sep;70(9):6044-53. doi: 10.1128/JVI.70.9.6044-6053.1996.
7
Tumor necrosis factor alpha inhibits entry of human immunodeficiency virus type 1 into primary human macrophages: a selective role for the 75-kilodalton receptor.肿瘤坏死因子α抑制人类免疫缺陷病毒1型进入原代人巨噬细胞：75千道尔顿受体的选择性作用。
J Virol. 1996 Nov;70(11):7388-97. doi: 10.1128/JVI.70.11.7388-7397.1996.
8
Abortive infection in HeLaCD4 cells by a primary HIV type 1 isolate: implications for differential host cell tropism.1型人类免疫缺陷病毒原代分离株在HeLaCD4细胞中的顿挫感染：对宿主细胞嗜性差异的影响
AIDS Res Hum Retroviruses. 1997 Jun 10;13(9):759-69. doi: 10.1089/aid.1997.13.759.
9
Human immunodeficiency virus infection and syncytium formation in HeLa cells expressing glycophospholipid-anchored CD4.在表达糖磷脂锚定CD4的HeLa细胞中人类免疫缺陷病毒感染与合胞体形成
J Virol. 1991 Jun;65(6):3276-83. doi: 10.1128/JVI.65.6.3276-3283.1991.
10
Human immunodeficiency virus type 1 Vpu protein induces degradation of chimeric envelope glycoproteins bearing the cytoplasmic and anchor domains of CD4: role of the cytoplasmic domain in Vpu-induced degradation in the endoplasmic reticulum.1型人类免疫缺陷病毒Vpu蛋白诱导携带CD4胞质和锚定结构域的嵌合包膜糖蛋白降解：内质网中胞质结构域在Vpu诱导降解中的作用
J Virol. 1993 Sep;67(9):5538-49. doi: 10.1128/JVI.67.9.5538-5549.1993.

引用本文的文献

1
α-Synuclein fibrils enhance HIV-1 infection of human T cells, macrophages and microglia.α-突触核蛋白原纤维增强人类T细胞、巨噬细胞和小胶质细胞的HIV-1感染。
Nat Commun. 2025 Jan 18;16(1):813. doi: 10.1038/s41467-025-56099-z.
2
Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.APOBEC3F、APOBEC3G 和 APOBEC3H 在限制 HIV-2 中的差异活性。
J Mol Biol. 2022 Jan 30;434(2):167355. doi: 10.1016/j.jmb.2021.167355. Epub 2021 Nov 10.
3
Distinct Antiretroviral Mechanisms Elicited by a Viral Mutagen.

本文引用的文献

1
Different requirements for membrane fusion mediated by the envelopes of human immunodeficiency virus types 1 and 2.1型和2型人类免疫缺陷病毒包膜介导的膜融合的不同要求。
J Virol. 1993 Apr;67(4):2355-9. doi: 10.1128/JVI.67.4.2355-2359.1993.
2
A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
Virology. 1973 Apr;52(2):456-67. doi: 10.1016/0042-6822(73)90341-3.
3
Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.1型人类免疫缺陷病毒包膜糖蛋白的功能区
病毒诱变引发的独特抗逆转录病毒机制。
J Mol Biol. 2021 Sep 3;433(18):167111. doi: 10.1016/j.jmb.2021.167111. Epub 2021 Jun 18.
4
Cell Cultures for Virology: Usability, Advantages, and Prospects.病毒学中的细胞培养：可用性、优势与前景
Int J Mol Sci. 2020 Oct 27;21(21):7978. doi: 10.3390/ijms21217978.
5
Astrocytes as an HIV Reservoir: Mechanism of HIV Infection.作为HIV储存库的星形胶质细胞：HIV感染机制
Curr HIV Res. 2016;14(5):373-381. doi: 10.2174/1570162x14666161006121455.
6
Dual anti-HIV mechanism of clofarabine.氯法拉滨的双重抗HIV机制。
Retrovirology. 2016 Mar 24;13:20. doi: 10.1186/s12977-016-0254-0.
7
Tetraspanin CD63 is a regulator of HIV-1 replication.四跨膜蛋白CD63是HIV-1复制的调节因子。
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1184-98. eCollection 2015.
8
Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.HIV-1 前整合复合物的核易位依赖于 ADAM10 细胞内结构域。
Virology. 2014 Apr;454-455:60-6. doi: 10.1016/j.virol.2014.02.006. Epub 2014 Feb 22.
9
Characterization of permeability, stability and anti-HIV-1 activity of decitabine and gemcitabine divalerate prodrugs.地西他滨和吉西他滨二戊酸酯前药的渗透性、稳定性及抗HIV-1活性的表征
Antivir Chem Chemother. 2014 Dec 16;23(6):223-30. doi: 10.3851/IMP2682.
10
Activity of a novel combined antiretroviral therapy of gemcitabine and decitabine in a mouse model for HIV-1.新型联合抗逆转录病毒疗法吉西他滨和地西他滨在 HIV-1 小鼠模型中的活性。
Antimicrob Agents Chemother. 2012 Apr;56(4):1942-8. doi: 10.1128/AAC.06161-11. Epub 2012 Jan 23.
Science. 1987 Sep 11;237(4820):1351-5. doi: 10.1126/science.3629244.
4
Detection of a fusion peptide sequence in the transmembrane protein of human immunodeficiency virus.在人类免疫缺陷病毒跨膜蛋白中检测融合肽序列
Cell. 1987 Jul 31;50(3):327-8. doi: 10.1016/0092-8674(87)90485-5.
5
The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.T4基因编码艾滋病病毒受体，并在免疫系统和大脑中表达。
Cell. 1986 Nov 7;47(3):333-48. doi: 10.1016/0092-8674(86)90590-8.
6
Synthetic CD4 peptide derivatives that inhibit HIV infection and cytopathicity.抑制HIV感染和细胞病变效应的合成CD4肽衍生物。
Science. 1988 Aug 5;241(4866):712-6. doi: 10.1126/science.2969619.
7
Neutralizing antibodies to an immunodominant envelope sequence do not prevent gp120 binding to CD4.针对免疫显性包膜序列的中和抗体并不能阻止gp120与CD4结合。
J Virol. 1988 Nov;62(11):4195-200. doi: 10.1128/JVI.62.11.4195-4200.1988.
8
CD4 antigen-based antireceptor peptides inhibit infectivity of human immunodeficiency virus in vitro at multiple stages of the viral life cycle.基于CD4抗原的抗受体肽在病毒生命周期的多个阶段可抑制人类免疫缺陷病毒在体外的感染性。
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7139-43. doi: 10.1073/pnas.86.18.7139.
9
Human immunodeficiency virus infection studied in CD4-expressing human-murine T-cell hybrids.在表达CD4的人鼠T细胞杂交体中研究人类免疫缺陷病毒感染。
Virology. 1989 Feb;168(2):267-73. doi: 10.1016/0042-6822(89)90266-3.
10
Effects of anti-gp120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1.抗gp120单克隆抗体对1型人类免疫缺陷病毒env蛋白与CD4受体结合的影响。
J Virol. 1988 Oct;62(10):3695-702. doi: 10.1128/JVI.62.10.3695-3702.1988.