Chopin D K, Caruelle J P, Colombel M, Palcy S, Ravery V, Caruelle D, Abbou C C, Barritault D
Service d'Urologie, Hôpital Henri Mondor, Créteil, France.
J Urol. 1993 Oct;150(4):1126-30. doi: 10.1016/s0022-5347(17)35705-1.
Acidic fibroblast growth factor is a regulatory peptide involved in cell proliferation, differentiation and motility. We used a polyclonal antiserum raised against purified native bovine acidic fibroblast growth factor, with no cross-reactivity for basic fibroblast growth factor to detect acidic fibroblast growth factor in tissue extracts and urine samples by means of a competitive enzyme immunoassay. Histochemical analysis was also performed on 10 specimens of normal urothelium and 50 of bladder cancer. Acidic fibroblast growth factor immunoreactive material was found in normal urothelium (1.77 +/- 2 ng./gm. tissue) and was increased more than 10-fold in patients with transitional cell carcinoma of the bladder (20.36 +/- 12 ng./gm. tissue). Immunohistochemical analysis localized immunoreactivity in the epithelial compartment of bladder tumors. Acidic fibroblast growth factor was assayed in urine from 579 individuals comprising a control group (114) and patients with benign prostatic hypertrophy (133), carcinoma of the prostate (96) or transitional cell carcinoma of the bladder (236). There was a significant difference in the frequency of urinary acidic fibroblast growth factor detection among the patients with invasive transitional cell carcinoma, the control group (p < 0.001) and the patients with prostatic disease (p < 0.01). The sensitivity was 72% and the specificity was 91%. Furthermore, the frequency of acidic fibroblast growth factor detection by enzyme immunoassay in the urine and the intensity of immunostaining was correlated with the stage of the disease. These data strongly suggest that acidic fibroblast growth factor is a potential marker for bladder tumors that may be of use in the noninvasive followup of patients with bladder cancer. We present a simple and reliable enzyme immunoassay for the detection of acidic fibroblast growth factor in voided urine that might be useful to quantitate this marker.
酸性成纤维细胞生长因子是一种参与细胞增殖、分化和运动的调节肽。我们使用针对纯化的天然牛酸性成纤维细胞生长因子产生的多克隆抗血清（对碱性成纤维细胞生长因子无交叉反应性），通过竞争性酶免疫测定法检测组织提取物和尿液样本中的酸性成纤维细胞生长因子。还对10例正常尿路上皮标本和50例膀胱癌标本进行了组织化学分析。在正常尿路上皮中发现了酸性成纤维细胞生长因子免疫反应性物质（1.77±2 ng/克组织），在膀胱移行细胞癌患者中增加了10倍以上（20.36±12 ng/克组织）。免疫组织化学分析将免疫反应性定位在膀胱肿瘤的上皮区室。对包括对照组（114例）和良性前列腺增生患者（133例）、前列腺癌患者（96例）或膀胱移行细胞癌患者（236例）在内的579名个体的尿液进行了酸性成纤维细胞生长因子检测。侵袭性膀胱移行细胞癌患者、对照组（p<0.001）和前列腺疾病患者（p<0.01）尿液中酸性成纤维细胞生长因子检测频率存在显著差异。敏感性为72%，特异性为91%。此外，通过酶免疫测定法在尿液中检测酸性成纤维细胞生长因子的频率和免疫染色强度与疾病分期相关。这些数据强烈表明，酸性成纤维细胞生长因子是膀胱肿瘤的潜在标志物，可能用于膀胱癌患者的无创随访。我们提出了一种简单可靠的酶免疫测定法，用于检测晨尿中的酸性成纤维细胞生长因子，这可能有助于定量该标志物。
