Tsuchiya H, el-Sonbaty S S, Watanabe M, Suzushima H, Asou N, Murakami T, Takeda T, Shimosaka A, Takatsuki K, Matsuda I
Department of Pediatrics, Kumamoto University School of Medicine, Japan.
Leuk Res. 1993 Sep;17(9):809-13. doi: 10.1016/0145-2126(93)90116-3.
We examined myeloid characteristics of myeloid-antigen-positive (My+) and -negative (My-) B-precursor acute lymphoblastic leukemia (ALL) blast cells. Immunophenotyping before and after culture, rearrangements of immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) genes, stimulation of DNA synthesis with granulocyte colony-stimulating factor (G-CSF), and G-CSF binding assay were performed. Of My+ ALL blasts, the immunophenotypic staging as B-precursor ALL and rearrangements of IgH and TCR-beta, gamma and delta genes did not differ from findings in My- ALL blasts. Stimulated with G-CSF, cells from one My+ ALL and from one My- ALL patients showed enhancement of DNA synthesis and expression of CD11b and CD13, respectively. G-CSF binding was observed in blasts from 3 My+ ALL patients and one My- ALL child. After culture, blasts from My- ALL children expressed CD13 but showed neither enhanced DNA synthesis with G-CSF nor G-CSF binding. Thus, it would appear that (i) My+ and My- adult ALL blasts are at the same stage of differentiation; (ii) some My+ adult ALL blasts have phenotypic and functional myeloid characteristics; and (iii) induction of CD13 expression in My- ALL after in vitro culture does not correlate with other myeloid characteristics.
我们检测了髓系抗原阳性（My+）和阴性（My-）的B前体急性淋巴细胞白血病（ALL）原始细胞的髓系特征。进行了培养前后的免疫表型分析、免疫球蛋白重链（IgH）和T细胞受体（TCR）基因重排、粒细胞集落刺激因子（G-CSF）刺激DNA合成以及G-CSF结合试验。在My+ ALL原始细胞中，作为B前体ALL的免疫表型分期以及IgH和TCR-β、γ和δ基因重排与My- ALL原始细胞的结果并无差异。用G-CSF刺激后，来自一名My+ ALL患者和一名My- ALL患者的细胞分别显示出DNA合成增强以及CD11b和CD13表达增强。在3名My+ ALL患者和1名My- ALL儿童的原始细胞中观察到G-CSF结合。培养后，My- ALL儿童的原始细胞表达CD13，但用G-CSF刺激时既未显示DNA合成增强，也未显示G-CSF结合。因此，似乎（i）My+和My-成人ALL原始细胞处于相同的分化阶段；（ii）一些My+成人ALL原始细胞具有表型和功能性髓系特征；（iii）体外培养后My- ALL中CD13表达的诱导与其他髓系特征无关。
