Edwards A J, Moon E Y, Anderson D, McGregor D B
BIBRA Toxicology International, Carshalton, Surrey, UK.
Mutat Res. 1993 Oct;289(2):139-44. doi: 10.1016/0027-5107(93)90063-l.
Assessment of genotoxicity in cultured cells or in experimental animals through the measurement of sister-chromatid exchanges (SCEs) commonly requires their simultaneous exposure to both the test agent and bromodeoxyuridine (BrdUrd). This dual exposure could lead to modified responses because of either synergistic or antagonistic interactions. Differences in protocol may also have their effect. There is, for example, time for DNA repair to take place in protocols in which there is separate exposure to the test agent and BrdUrd, such as human genetic monitoring studies. In this study, human lymphocyte cultures have been used to investigate the effect of the duration of simultaneous exposure to the mutagen methyl methanesulphonate (MMS) and to BrdUrd on SCE incidence. There was a direct relationship between SCE frequency and the time of simultaneous exposure to MMS and BrdUrd that was not dependent on either the total culture time or the total time of exposure to BrdUrd. This suggestion of an interaction between MMS and BrdUrd in inducing SCEs has important implications for the interpretation of SCE data in both experimental and human monitoring studies.
通过测量姐妹染色单体交换（SCE）来评估培养细胞或实验动物中的遗传毒性，通常需要它们同时暴露于受试物和溴脱氧尿苷（BrdUrd）。这种双重暴露可能会由于协同或拮抗相互作用而导致反应改变。实验方案的差异也可能有影响。例如，在诸如人类遗传监测研究等受试物和BrdUrd分开暴露的实验方案中，存在DNA修复的时间。在本研究中，已使用人类淋巴细胞培养物来研究同时暴露于诱变剂甲磺酸甲酯（MMS）和BrdUrd的持续时间对SCE发生率的影响。SCE频率与同时暴露于MMS和BrdUrd的时间之间存在直接关系，这与总培养时间或暴露于BrdUrd的总时间无关。MMS和BrdUrd在诱导SCE方面存在相互作用的这一迹象，对实验研究和人类监测研究中SCE数据的解释具有重要意义。
