Imai T, Fujimaki H, Abe T, Befus D
Department of ENT, Ohmori Red Cross Hospital, Tokyo, Japan.
Int Arch Allergy Immunol. 1993;102(1):26-32. doi: 10.1159/000236547.
We isolated RNA from in vivo-derived rat peritoneal (PMC) and intestinal mucosal mast cells (IMMC) and assessed the in vitro translation products of these two mast cell types using rabbit reticulocyte lysate and wheat germ systems. The translated polypeptides were analyzed by one- and two-dimensional gel electrophoresis. Fluorographic analyses established that polypeptides with a spectrum of molecular size and pI were produced by both PMC and IMMC. Immunoprecipitation studies with anti-rat mast cell protease (RMCP) I antibodies detected a band at 31 kD from products of PMC RNA assessed by one-dimensional electrophoresis, whereas anti-RMCP II antibodies precipitated a band of 27 kD from products of IMMC RNA. This is the first report of translation products of IMMC and of the translated precursors of RMCP I and RMCP II.
我们从体内来源的大鼠腹膜肥大细胞（PMC）和肠黏膜肥大细胞（IMMC）中分离RNA，并使用兔网织红细胞裂解物和麦胚系统评估这两种肥大细胞类型的体外翻译产物。通过一维和二维凝胶电泳分析翻译后的多肽。荧光显影分析表明，PMC和IMMC均产生了具有一系列分子大小和等电点的多肽。用抗大鼠肥大细胞蛋白酶（RMCP）I抗体进行的免疫沉淀研究在一维电泳评估的PMC RNA产物中检测到一条31 kD的条带，而抗RMCP II抗体从IMMC RNA产物中沉淀出一条27 kD的条带。这是关于IMMC翻译产物以及RMCP I和RMCP II翻译前体的首次报道。
