Li J Y, Dahlström A B
Department of Anatomy and Cell Biology, University of Göteborg, Sweden.
Brain Res Dev Brain Res. 1993 Aug 20;74(2):269-82. doi: 10.1016/0165-3806(93)90013-z.
GAP 43 in nerve terminal structures of rat skeletal muscles, was investigated during postnatal development using immunofluorescence and confocal laser scanning microscopy. Comparison with synaptophysin, synapsin, SV2, CGRP, SP and NF was done in double immunoincubation studies. GAP 43-like immunoreactivity (LI) was demonstrated in preterminal axons and motor endplates in all age groups (from E18 to adult), although the intensity of immunofluorescence was considerably higher in the younger rats. The outgrowing nerve sprouts in E18 muscles were strongly GAP 43-positive. The intensity decreased with increasing age, but even in adult animals GAP 43-LI was present in some p38- or SV2-positive endplates. GAP 43-LI was also present in muscle spindles and preterminal nerve branches, and likewise decreased with age. Perivascular nerve terminals (around arteries mainly) were, however, strong in GAP 43-LI during both development and adulthood. GAP 43-LI was strong, and present in both small and large granules. SP-LI was observed in a few thin, presumably sensory, axons around vessels, which also contained a few GAP 43-positive large granules. Most of the strongly GAP 43-positive terminals around vessels were probably autonomic postganglionic terminals. The results suggest that GAP 43, in addition to development and regeneration, may play a significant role also in normal adult rats, especially in perivascular nerve terminals, possibly connected with a high potential for plasticity in this kind of nerve terminals.
利用免疫荧光和共聚焦激光扫描显微镜，对大鼠骨骼肌神经终末结构中的GAP 43在出生后发育过程中进行了研究。在双重免疫孵育研究中，将其与突触素、突触结合蛋白、SV2、降钙素基因相关肽、P物质和神经丝进行了比较。在所有年龄组（从胚胎18天到成年）的终末前轴突和运动终板中均显示出GAP 43样免疫反应性（LI），尽管在幼鼠中免疫荧光强度明显更高。胚胎18天肌肉中生长的神经芽强烈呈GAP 43阳性。强度随年龄增长而降低，但即使在成年动物中，GAP 43-LI也存在于一些p38或SV2阳性终板中。GAP 43-LI也存在于肌梭和终末前神经分支中，同样随年龄降低。然而，血管周围神经终末（主要围绕动脉）在发育和成年期GAP 43-LI均较强。GAP 43-LI较强，存在于小颗粒和大颗粒中。在血管周围一些较细的、可能是感觉性的轴突中观察到P物质-LI，这些轴突中也含有一些GAP 43阳性大颗粒。血管周围大多数强烈GAP 43阳性的终末可能是自主神经节后终末。结果表明，GAP 43除了在发育和再生中起作用外，在正常成年大鼠中也可能起重要作用，尤其是在血管周围神经终末，这可能与这类神经终末的高可塑性潜力有关。
