线粒体内膜的pH依赖性阴离子传导通道受到锌离子的强烈抑制。

The pH-dependent anion-conducting channel of the mitochondrial inner membrane is potently inhibited by zinc ions.

作者信息

Selwyn M J, Ng L T, Choo H L

机构信息

Department of Biochemistry, Faculty of Medicine, National University of Singapore.

出版信息

FEBS Lett. 1993 Sep 27;331(1-2):129-33. doi: 10.1016/0014-5793(93)80311-h.

DOI:10.1016/0014-5793(93)80311-h

PMID:7691656

Abstract

Zinc is a potent reversible inhibitor of the pH-dependent anion-conducting channel in the mitochondrial inner membrane, 50% inhibition was produced by 1.5 microM added Zn2+ at which point free Zn2+ was < or = 10(-8) M. Inhibition by Zn2+ is rapid but can be prevented or rapidly reversed by excess EDTA. Concentrations of Zn2+ higher than 4 microM caused reversal of inhibition to a variable extent depending on the anion. Under these conditions Zn2+ did not inhibit ribose entry, the phosphate transporter, or the pH-insensitive component of the NO3- uniport.

摘要

锌是线粒体内膜中pH依赖性阴离子传导通道的一种强效可逆抑制剂，添加1.5微摩尔Zn2+时产生50%的抑制作用，此时游离Zn2+浓度≤10^(-8) M。Zn2+的抑制作用迅速，但过量的乙二胺四乙酸（EDTA）可防止或迅速逆转这种抑制作用。高于4微摩尔的Zn2+浓度会导致抑制作用不同程度的逆转，具体取决于阴离子。在这些条件下，Zn2+不抑制核糖进入、磷酸转运体或硝酸根单向转运体的pH不敏感成分。