pp59fyn和pp62c-yes在SH-SY5Y神经母细胞瘤生长锥中富集，但不与与pp60c src和pp60c-srcN形成复合物的38 kDa蛋白相关联。

pp59fyn and pp62c-yes are enriched in SH-SY5Y neuroblastoma growth cones but do not associate to the 38 kDa protein which complexes with pp60c src and pp60c-srcN.

作者信息

Meyerson G, Påhlman S

机构信息

Department of Pathology, University of Uppsala, University Hospital, Sweden.

出版信息

FEBS Lett. 1993 Oct 11;332(1-2):27-30. doi: 10.1016/0014-5793(93)80475-a.

DOI:10.1016/0014-5793(93)80475-a

PMID:7691658

Abstract

The tyrosine-specific kinases pp60c-src and pp60c-srcN (pp60src) are slightly enriched and activated in growth cones isolated from neuronally differentiating SH-SY5Y neuroblastoma cells. In the growth cones the two src isoforms are associated with a 38 kDa protein. In this report, we have compared the subcellular distribution of pp59fyn and pp62c-yes with that of pp60src in differentiating SH-SY5Y cells. Like pp60src, the other two tyrosine kinases were slightly enriched and activated in the growth cones as compared to the levels in the cell bodies. The kinase activities were 3- to 4-times higher in growth cones than in cell bodies. However, only pp60src formed a complex with the 38 kDa protein while immunoprecipitation of pp59fyn brought down an additional protein of 90 kDa. This may suggest that these related tyrosine kinases have different substrates and in part mediate different cellular responses in the growth cones of differentiating SH-SY5Y cells.

摘要

酪氨酸特异性激酶pp60c-src和pp60c-srcN（pp60src）在从神经分化的SH-SY5Y神经母细胞瘤细胞分离的生长锥中略有富集并被激活。在生长锥中，这两种src亚型与一种38 kDa的蛋白质相关联。在本报告中，我们比较了pp59fyn和pp62c-yes与pp60src在分化的SH-SY5Y细胞中的亚细胞分布。与pp60src一样，与细胞体中的水平相比，其他两种酪氨酸激酶在生长锥中也略有富集并被激活。生长锥中的激酶活性比细胞体中的高3至4倍。然而，只有pp60src与38 kDa的蛋白质形成复合物，而pp59fyn的免疫沉淀则沉淀出一种额外的90 kDa蛋白质。这可能表明这些相关的酪氨酸激酶具有不同的底物，并在一定程度上介导了分化的SH-SY5Y细胞生长锥中的不同细胞反应。