Bickmore W A, Longbottom D, Oghene K, Fletcher J M, van Heyningen V
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.
Genomics. 1993 Jul;17(1):129-35. doi: 10.1006/geno.1993.1293.
The human CD59 gene encodes a cell surface antigen detected by MEM43 and other antibodies. It has homology to the mouse Ly-6 genes that map on mouse chromosome 15 and are involved in lymphocyte signal transduction. CD59 may play a role in protecting against complement-mediated lysis. The human CD59 gene had been previously localized to 11p13-p14. We expected, on the basis of synteny arguments, that CD59 would map in 11p14. However, we have precisely localized the human CD59 gene to band p13 of chromosome 11 by somatic cell genetics and by pulsed-field gel electrophoresis; indeed the gene is often deleted in WAGR individuals. This region of chromosome 11 is syntenic with mouse chromosome 2. This suggests that CD59 is not a homolog of the mouse Ly-6 genes on mouse chromosome 15, but rather is a related gene. The human CD59 gene is shown to map within 500 kb of another cell surface marker, MIC11, the gene for which has not been cloned. This localization and the results of immunoprecipitation experiments suggest that the CD59 gene could encode the MIC11 antigen; alternatively this region of 11p13 may contain a cluster of genes encoding cell surface molecules.
人类CD59基因编码一种可被MEM43及其他抗体检测到的细胞表面抗原。它与定位于小鼠15号染色体且参与淋巴细胞信号转导的小鼠Ly-6基因具有同源性。CD59可能在防止补体介导的细胞溶解中发挥作用。人类CD59基因先前被定位于11p13-p14。基于同线性的观点，我们预期CD59会定位于11p14。然而，我们通过体细胞遗传学和脉冲场凝胶电泳将人类CD59基因精确地定位于11号染色体的p13带；实际上该基因在WAGR个体中常被缺失。11号染色体的这个区域与小鼠2号染色体同线。这表明CD59并非小鼠15号染色体上小鼠Ly-6基因的同源物，而是一个相关基因。人类CD59基因被证明定位于另一个细胞表面标志物MIC11的500 kb范围内，MIC11的基因尚未被克隆。这种定位以及免疫沉淀实验的结果表明，CD59基因可能编码MIC11抗原；或者11p13的这个区域可能包含一组编码细胞表面分子的基因。
