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Fatty acid acylation of RNase A using reversed micelles as microreactors.

作者信息

Robert S, Domurado D, Thomas D, Chopineau J

机构信息

Laboratoire de Technologie Enzymatique, URA CNRS 1442, Université de Technologie de Compiègne, France.

出版信息

Biochem Biophys Res Commun. 1993 Oct 15;196(1):447-54. doi: 10.1006/bbrc.1993.2270.

DOI:10.1006/bbrc.1993.2270
PMID:7692851
Abstract

A water soluble protein, RNAse A, was fatty-acylated using AOT reversed micelles in 2,2,4-trimethyl pentane as microreactors and myristoyl chloride as reagent. Artificial attachment of lipid molecules to this protein was performed for different hydration degrees by changing Wo = [water]/ [AOT], the parameter which controls the microreactor size. The chemically modified protein was monitored using reverse phase HPLC and characterized by HPLC, free amino groups titration, and electrophoresis. An RNase A/myristoyl chloride ratio of 1:4 (mol/mol) at Wo = 7 was found to give 60% of modified protein.

摘要

相似文献

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Fatty acid acylation of RNase A using reversed micelles as microreactors.
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