Yamada Y, Kuboi R, Komasawa I
Department of Chemical Engineering, Osaka University, Japan.
Biotechnol Prog. 1993 Sep-Oct;9(5):468-72. doi: 10.1021/bp00023a005.
Modification of AOT reverse micellar systems with various alkyl glucosides and nonionic surfactants was attempted in order to improve the activity to Chromobacterium viscosum lipase. Tweens and Tritons, having the poly(oxyethylene) chain, have been shown to be solubilized at the oil-water interface of AOT micelles. Alkyl glucosides were also solubilized at the interface. These additives form mixed micelles with AOT and increase the concentration of the micelles. In contrast, Spans were found to be solubilized in the micro water pool of AOT micelles. Addition of nonionic surfactants decreased the hydrophobicity of the reverse micellar systems. Improvement of the lipase activity was achieved by the addition of nonionic surfactants, because of suppression of the hydrophobic and electrostatic interaction between AOT and lipase. The addition of nonionic surfactant having a poly(oxyethylene) chain has been shown to effectively increase the activity of the lipase in reverse micelles.
为了提高对粘性色杆菌脂肪酶的活性，尝试用各种烷基糖苷和非离子表面活性剂对AOT反胶束体系进行改性。已表明具有聚（氧乙烯）链的吐温类和曲通类可溶解于AOT胶束的油水界面。烷基糖苷也溶解于该界面。这些添加剂与AOT形成混合胶束并增加胶束浓度。相比之下，发现司盘类溶解于AOT胶束的微水池中。添加非离子表面活性剂降低了反胶束体系的疏水性。由于抑制了AOT与脂肪酶之间的疏水和静电相互作用，通过添加非离子表面活性剂实现了脂肪酶活性的提高。已表明添加具有聚（氧乙烯）链的非离子表面活性剂可有效提高反胶束中脂肪酶的活性。
