Cahill M R, Macey M G, Newland A C
Department of Haematology, Royal London Hospital.
Br J Haematol. 1993 Jul;84(3):527-9. doi: 10.1111/j.1365-2141.1993.tb03112.x.
Platelet activation in vivo is important in the pathogenesis of thrombosis. Accurate measurement is difficult due to artefactual in vitro preparation related activation. This can be overcome by using whole blood techniques, such as flow cytometry. However, there is little consensus on methods of platelet preparation, procedures for use of fixation, or the optimal types/amounts of fixative. The use of unfixed platelets has received little attention. We describe a series of experiments comparing platelet activation antigen expression detected by flow cytometry in fixed and unfixed samples. Formaldehyde increased the expression of both CD62 and CD63. We recommend that fixation with formaldehyde should not be used when studying platelet activation.
体内血小板活化在血栓形成的发病机制中很重要。由于体外制备相关的人为激活，准确测量很困难。这可以通过使用全血技术（如流式细胞术）来克服。然而，关于血小板制备方法、固定剂使用程序或固定剂的最佳类型/用量，几乎没有共识。未固定血小板的使用很少受到关注。我们描述了一系列实验，比较了通过流式细胞术在固定和未固定样本中检测到的血小板活化抗原表达。甲醛增加了CD62和CD63的表达。我们建议在研究血小板活化时不应使用甲醛固定。
