Schmitt J, Pohl J, Stunnenberg H G
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Gene. 1993 Oct 15;132(2):267-71. doi: 10.1016/0378-1119(93)90206-i.
A cDNA encoding the homologue of the rabbit immunophilin p59 was cloned from a mouse NIH-3T3 cell line library. Antibodies were generated against the N-terminal fragment of the protein produced in bacteria. Western blotting experiments suggest that homologous proteins are present in several other cell lines tested. Production of mouse p59 using recombinant vaccinia viruses resulted in a protein with the expected size of 59 kDa that can interact with the recombinant glucocorticoid receptor, as shown by co-immunoprecipitation experiments.
从小鼠NIH-3T3细胞系文库中克隆出编码兔亲免素p59同源物的cDNA。针对细菌中产生的该蛋白的N端片段制备了抗体。蛋白质印迹实验表明，在所检测的其他几种细胞系中也存在同源蛋白。如共免疫沉淀实验所示，利用重组痘苗病毒生产小鼠p59，得到了预期大小为59 kDa的蛋白，该蛋白可与重组糖皮质激素受体相互作用。
