Yoshimoto K, Irizawa Y, Komura S
Department of Legal Medicine, Kyoto Prefectural University of Medicine, Japan.
Forensic Sci Int. 1993 Aug;60(3):183-8. doi: 10.1016/0379-0738(93)90237-5.
Brain microdialysis was used to monitor changes in extracellular dopamine (DA), serotonin (5-HT), and their metabolite levels in the rat striatum at death by cervical dislocation. Maximum respective 450-fold and 150-fold increases in the extracellular output of DA and 5-HT were observed within the first 30 min of death. DA and 5-HT outputs remained elevated over the following 2 h at levels about 100-fold and 50-fold above pre-death values, respectively. In contrast with monoamine outputs, the outputs of the DA metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), and the 5-HT metabolite, 5-hydroxyindoleacetic acid (5-HIAA), rapidly decreased by 10% and 20%, respectively 1 h after death. 5-Hydroxytryptophol (5-HTOL) gradually decreased after death. Before death both the extracellular DOPAC/DA and 5-HIAA/5-HT ratios were about 400; after death these ratios dropped to 0.56 and 4.0, respectively at 30 min. These observations suggested that regulation of neurotransmitter releases through the neuronal membrane and metabolisms in the rat striatum were seriously disrupted at death. This finding may be helpful in the determination of death in the field of forensic medicine.
采用脑微透析技术监测大鼠颈椎脱臼处死时纹状体内细胞外多巴胺（DA）、5-羟色胺（5-HT）及其代谢产物水平的变化。在死亡后的最初30分钟内，观察到细胞外DA和5-HT的输出量分别最大增加了450倍和150倍。在随后的2小时内，DA和5-HT的输出量一直保持升高，分别比死亡前的值高出约100倍和50倍。与单胺输出量不同，DA代谢产物3,4-二羟基苯乙酸（DOPAC）和5-HT代谢产物5-羟吲哚乙酸（5-HIAA）的输出量在死亡1小时后分别迅速下降了10%和20%。5-羟色胺醇（5-HTOL）在死亡后逐渐下降。死亡前，细胞外DOPAC/DA和5-HIAA/5-HT的比值均约为400；死亡后30分钟时，这些比值分别降至0.56和4.0。这些观察结果表明，大鼠纹状体内通过神经元膜的神经递质释放调节和代谢在死亡时受到严重破坏。这一发现可能有助于法医学领域的死亡判定。
