Kobayashi N, Taniguchi K, Urasawa T, Urasawa S
Department of Hygiene, Sapporo Medical College, Japan.
J Virol Methods. 1993 Sep;44(1):25-34. doi: 10.1016/0166-0934(93)90004-b.
Anti-VP4 and anti-VP7 neutralizing monoclonal antibodies (N-MAbs) were used to select efficiently antigenic mosaic reassortants of rotavirus whose VP4 and VP7 are derived from parental strains having different antigenic specificities of VP4 and VP7. Under the selective pressure of anti-simian rotavirus (SA11) VP7 N-MAb and anti-human rotavirus (Wa or S2) VP4 N-MAb, all the clones isolated after mixed infection with SA11 and human rotavirus were found to be reassortants having VP4 of SA11 and VP7 of Wa or S2 by serological and genomic analyses. Two reassortants isolated from the mixed infection with SA11 and S2 had a segment whose different migration in polyacrylamide gel was different from those of any RNA segments of the parent strains, suggesting the occurrence of rearrangement in the process of reassortment.
抗VP4和抗VP7中和单克隆抗体（N-MAbs）被用于高效筛选轮状病毒的抗原嵌合重配体，其VP4和VP7来源于具有不同VP4和VP7抗原特异性的亲本菌株。在抗猴轮状病毒（SA11）VP7 N-MAb和抗人轮状病毒（Wa或S2）VP4 N-MAb的选择压力下，通过血清学和基因组分析发现，SA11与人轮状病毒混合感染后分离出的所有克隆均为重配体，其VP4来自SA11，VP7来自Wa或S2。从SA11与S2的混合感染中分离出的两个重配体有一个片段，其在聚丙烯酰胺凝胶中的迁移情况与亲本菌株的任何RNA片段都不同，这表明在重配过程中发生了重排。
