Finke J, Ternes P, Lange W, Mertelsmann R, Dölken G
Department of Hematology and Oncology, University of Freiburg, Germany.
Leukemia. 1993 Nov;7(11):1852-7.
Interleukin 10 (IL-10) is a strong B-cell-activating factor. Since murine Ly1-positive peritoneal or lymphoma B cells strongly express IL-10, we examined malignant cells from patients with acute and chronic leukemias by reverse transcriptase polymerase chain reaction (RT-PCR) for the expression of IL-10 mRNA. High expression was found in three out of four samples from CD10+, CD5- common acute lymphoblastic leukemia (cALL) cells, whereas T-ALL samples generally did not contain IL-10 mRNA. In contrast to the murine system, only low levels of IL-10 expression were seen in 10 out of 11 samples from patients with CD5+ CLL. Myeloid derived cell lines were negative for IL-10. Epstein Barr virus (EBV) positive and negative Burkitt's lymphoma (BL) cell lines showed heterogenous IL-10 expression: BL cell lines with a nonactivated germinal center phenotype (CD10+, CD77+, CD23-, CD30-, CDw70-) expressed little or no IL-10, whereas EBV-positive and negative BL cell lines with an activated phenotype (CD77-, CD23+, CD30+, CDw70+) expressed easily detectable amounts of IL-10 mRNA. The highest expression of IL-10 was found in EBV-immortalized lymphoblastoid cell lines (LCL). Upon superinfection with non-defective EBV, the EBV-negative cell line BL 41 up-regulated IL-10 expression. Thus, in vivo IL-10 expression is not restricted to cells showing a specific (CD5+) phenotype. IL-10 may play an important role in c-ALL. The expression of IL-10 in BL cell lines is correlated to an activated phenotype in vitro and is independent of the EBV carrier status. EBV can induce IL-10 expression.
白细胞介素10（IL-10）是一种强大的B细胞激活因子。由于小鼠Ly1阳性腹膜或淋巴瘤B细胞强烈表达IL-10，我们通过逆转录酶聚合酶链反应（RT-PCR）检测了急性和慢性白血病患者的恶性细胞中IL-10 mRNA的表达。在来自CD10 +、CD5 - 普通急性淋巴细胞白血病（cALL）细胞的四个样本中，有三个发现高表达，而T-ALL样本通常不含IL-10 mRNA。与小鼠系统不同，在11例CD5 + 慢性淋巴细胞白血病（CLL）患者的样本中，只有10例检测到低水平的IL-10表达。髓系来源的细胞系IL-10呈阴性。爱泼斯坦 - 巴尔病毒（EBV）阳性和阴性的伯基特淋巴瘤（BL）细胞系表现出异质性的IL-10表达：具有未活化生发中心表型（CD10 +、CD77 +、CD23 -、CD30 -、CDw70 -）的BL细胞系几乎不表达或不表达IL-10，而具有活化表型（CD77 -、CD23 +、CD30 +、CDw70 +）的EBV阳性和阴性BL细胞系表达易于检测到的IL-10 mRNA量。IL-10的最高表达见于EBV永生化的淋巴母细胞系（LCL）。用无缺陷的EBV超感染后，EBV阴性细胞系BL 41上调了IL-10表达。因此，体内IL-10的表达并不局限于表现出特定（CD5 +）表型的细胞。IL-10可能在cALL中起重要作用。BL细胞系中IL-10的表达与体外活化表型相关，且与EBV携带状态无关。EBV可诱导IL-10表达。
