Lin R H, Wu L J, Lee C H, Lin-Shiau S Y
Department of Pharmacology, Chung Shan Medical and Dental College, Taichung, Taiwan.
Mutat Res. 1993 Nov;319(3):197-203. doi: 10.1016/0165-1218(93)90079-s.
The effects of uranyl nitrate (UO2+2) on viability, cell cycle kinetics (CCK), micronuclei (MN), chromosome aberrations (CA), and sister-chromatid exchanges (SCE) in Chinese hamster ovary (CHO) cells were investigated. Uranyl nitrate decreased the viability of CHO cells in a dose-related fashion. The concentration for 50% inhibition (IC50) of uranyl nitrate on viability was 0.049 mM. Uranyl nitrate at concentrations ranging from 0.01 to 0.3 mM decreased CCK and increased frequencies of MN and SCE. CA were also significantly augmented by uranyl nitrate. This finding indicates that uranyl nitrate has the property of causing genotoxicity and cytotoxicity in CHO cells. It appears that this cytogenetic toxicity of uranyl nitrate provides a biological basis for the potential teratogenic effect of uranium on developing fetal mice.
研究了硝酸铀酰（UO2+2）对中国仓鼠卵巢（CHO）细胞活力、细胞周期动力学（CCK）、微核（MN）、染色体畸变（CA）和姐妹染色单体交换（SCE）的影响。硝酸铀酰以剂量相关的方式降低了CHO细胞的活力。硝酸铀酰对活力的50%抑制浓度（IC50）为0.049 mM。浓度范围为0.01至0.3 mM的硝酸铀酰降低了CCK，并增加了MN和SCE的频率。硝酸铀酰也显著增加了CA。这一发现表明硝酸铀酰具有在CHO细胞中引起遗传毒性和细胞毒性的特性。看来硝酸铀酰的这种细胞遗传学毒性为铀对发育中的胎鼠的潜在致畸作用提供了生物学基础。
