Mulholland M W, Simeone D M
Department of Surgery, University of Michigan, Ann Arbor.
Am J Surg. 1993 Nov;166(5):552-6. doi: 10.1016/s0002-9610(05)81152-3.
Prostaglandins are widely distributed within the gastrointestinal tract and have important effects on gastrointestinal motility. Although actions upon gastrointestinal smooth muscle have been demonstrated for prostaglandins (PG), neural mechanisms may also be important. The effects of PGE2 on acetylcholine (ACh) release were studied using guinea pig myenteric plexus neurons in primary culture. PGE stimulated the release of ACh in a dose-dependent manner, with a median effective dose (ED50) of 0.8 x 10(-6) M. Involvement of L-type calcium channels was implied by the sensitivity of PGE2-stimulated ACh release to omega conotoxin or diltiazem. In addition, ACh release stimulated by PGE2 could be inhibited by co-incubation with dideoxyadenosine or Rp-cAMPS, agents that act upon cyclic adenosine monophosphate (cAMP)-dependent signaling pathways. PGE2 has direct effects upon myenteric neurons to stimulate cholinergic transmission.
前列腺素广泛分布于胃肠道内，对胃肠蠕动有重要影响。虽然已经证实前列腺素（PG）对胃肠平滑肌有作用，但神经机制可能也很重要。使用原代培养的豚鼠肠肌丛神经元研究了前列腺素E2（PGE2）对乙酰胆碱（ACh）释放的影响。PGE以剂量依赖性方式刺激ACh释放，半数有效剂量（ED50）为0.8×10（-6）M。PGE2刺激的ACh释放对ω-芋螺毒素或地尔硫䓬敏感，提示L型钙通道参与其中。此外，与双脱氧腺苷或Rp-cAMPS共同孵育可抑制PGE2刺激的ACh释放，这两种试剂作用于环磷酸腺苷（cAMP）依赖性信号通路。PGE2对肠肌神经元有直接作用，可刺激胆碱能传递。
