Germain P, Harbrioux G
Laboratoire de Biochimie, Faculté de Pharmacie, Limoges, France.
Anticancer Res. 1993 Sep-Oct;13(5A):1581-5.
The influence of bovine serum albumin on estradiol-17 beta growth and responsiveness of human mammary cancer MCF-7 cells was studied and compared to that of dextran-coated charcoal-treated fetal calf serum and other bovine serum proteins. To reveal the protein factor role, a culture defined medium was used. It was composed of minimal essential medium without phenol red, added with human iron saturated tranferrin (10 mg/ml) and porcine insulin (5 mg/ml). An estradiol-17 beta mitogenic effect was noticed only in the present of bovine serum albumin or of dextran-coated charcoal-treated fetal calf serum. A protein concentration increase provokes a greater responsiveness of MCF-7 cells submitted to an estrogenic treatment. The obtained results suggest that serum albumin participates in the estradiol-17 beta mode of action on the target cells and modulates its effect. The culture conditions appear essential for the study of the effect of estrogens and antiestrogens on the growth of estrogen-dependent cells.
研究了牛血清白蛋白对人乳腺癌MCF - 7细胞中雌二醇 - 17β生长及反应性的影响，并将其与葡聚糖包被活性炭处理的胎牛血清及其他牛血清蛋白的影响进行了比较。为揭示蛋白质因子的作用，使用了一种限定培养基。它由不含酚红的最低必需培养基组成，并添加了人铁饱和转铁蛋白（10毫克/毫升）和猪胰岛素（5毫克/毫升）。仅在存在牛血清白蛋白或葡聚糖包被活性炭处理的胎牛血清时，才观察到雌二醇 - 17β的促有丝分裂作用。蛋白质浓度的增加会使接受雌激素处理的MCF - 7细胞产生更大的反应性。所得结果表明，血清白蛋白参与了雌二醇 - 17β对靶细胞的作用模式并调节其效应。培养条件对于研究雌激素和抗雌激素对雌激素依赖性细胞生长的影响似乎至关重要。
