Inglot A D, Zielińska-Jenczylik J, Piasecki E
Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1993;41(1):73-80.
Tołpa Torf Preparation (TTP) is a natural immunomodulating drug registered in Poland for use in humans. TTP is a biologically active low molecular weight fraction of an extract from peat containing organic substances, primary bound sugars, amino-acids, uronic and huminic acids and mineral salts. The toxicity of TTP is remarkably low, eg. cytotoxicity (CD50) for human peripheral blood leukocytes (PBL) is 1-9 mg/ml. We have discovered that TTP is an interferon (IFN) and tumor necrosis factor (TNF) inducer in human PBL. The IFN and TNF response of the PBL cultures was dose dependent. The optimal concentration of TTP for IFN or TNF response was 10-100 micrograms/ml. The cytokines stimulated by TTP were IFN-gamma, IFN-alpha and TNF-alpha. Ten commercial batches of TTP have been found to be active as cytokines inducers although variations in their activities were observed. On the other hand, 8 batches of TTP rejected by the producer because of the inadequate immunostimulating activity determined in mice, were found to be significantly less active than the commercial preparations. Over 115 buffy coats from the individual blood donors were used to prepare PBL cultures for this study. Approximately 20% of the PBL cultures were unresponsive to TTP. The IFN and TNF response of PBL to other inducers: phytohemagglutinin (PHA) or lipopolysaccharides (LPS) also varied. Whereas only 7% of PBL could not be stimulated by PHA, as much as 20-50% of PBL failed to produce IFN or TNF when treated with LPS. We suggest that TTP may have clinically useful activities connected with the capacity of stimulation of IFNs and TNF production.
泥炭制剂（TTP）是一种在波兰注册用于人类的天然免疫调节药物。TTP是泥炭提取物的一种具有生物活性的低分子量组分，含有有机物质、主要结合糖、氨基酸、糖醛酸和腐殖酸以及矿物盐。TTP的毒性非常低，例如，对人外周血白细胞（PBL）的细胞毒性（CD50）为1 - 9毫克/毫升。我们发现TTP是人类PBL中的干扰素（IFN）和肿瘤坏死因子（TNF）诱导剂。PBL培养物的IFN和TNF反应呈剂量依赖性。TTP诱导IFN或TNF反应的最佳浓度为10 - 100微克/毫升。TTP刺激产生的细胞因子为IFN - γ, IFN - α和TNF - α。已发现十批市售TTP作为细胞因子诱导剂具有活性，尽管观察到它们的活性存在差异。另一方面，由于在小鼠中测定的免疫刺激活性不足而被生产商拒收的八批TTP，其活性明显低于市售制剂。本研究使用了来自个体献血者的超过115份血沉棕黄层来制备PBL培养物。大约20%的PBL培养物对TTP无反应。PBL对其他诱导剂：植物血凝素（PHA）或脂多糖（LPS）的IFN和TNF反应也有所不同。虽然只有7%的PBL不能被PHA刺激，但在用LPS处理时，多达20 - 50%的PBL未能产生IFN或TNF。我们认为TTP可能具有与刺激IFN和TNF产生能力相关的临床有用活性。
