Rapak A, Szajerka G, Maćkiewicz Z, Kupryszewski G, Szewczuk A
Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Wrocław, Poland.
Arch Immunol Ther Exp (Warsz). 1993;41(2):111-4.
Three synthetic peptides corresponding to amino acid sequences of two human chorionic gonadotropin (hCG) subunits were conjugated to thyroglobulin and used for immunization of rabbits. The highest antibody concentration was found in antisera from rabbits immunized with the peptide corresponding to 122-145 sequence of the hormone beta-subunit. Specificity of antisera and immunoaffinity purified rabbit antibodies were studied. Antibody anti-122-145-beta hCG with antibody anti-beta hCG conjugated to peroxidase were suitable for sensitive and specific enzyme immunoassay of hCG and beta hCG. Good correlation was noted between assay of the hormone in sera of patients with trophoblastic diseases or in urine of pregnant women by ELISA with the rabbit antibody pair and by commercial kit or by ELISA with two monoclonal antibodies.
将对应于人绒毛膜促性腺激素（hCG）两个亚基氨基酸序列的三种合成肽与甲状腺球蛋白偶联，并用于免疫兔子。在用对应于激素β亚基122 - 145序列的肽免疫的兔子抗血清中发现了最高的抗体浓度。研究了抗血清和免疫亲和纯化的兔抗体的特异性。抗122 - 145-β hCG抗体与与过氧化物酶偶联的抗β hCG抗体适用于hCG和β hCG的灵敏且特异的酶免疫测定。通过使用兔抗体对的ELISA法与商业试剂盒或使用两种单克隆抗体的ELISA法对滋养层疾病患者血清或孕妇尿液中的激素进行测定时，发现相关性良好。
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