人神经上皮瘤细胞系SK-N-MCIXC中胆囊收缩素mRNA对第二信使激活剂的反应调节。

Regulation of CCK mRNA in the human neuroepithelioma cell line SK-N-MCIXC in response to second messenger activators.

作者信息

Mania-Farnell B L, Merrill B J, Konings P N, Davis T P

机构信息

Department of Pharmacology, College of Medicine, University of Arizona, Tucson 85724.

出版信息

FEBS Lett. 1993 Nov 29;335(1):65-8. doi: 10.1016/0014-5793(93)80440-6.

DOI:10.1016/0014-5793(93)80440-6

PMID:7694875

Abstract

Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC. The cells were treated with the phosphodiesterase inhibitor isobutyl-methylxanthine and the tumor promoting phorbol ester, phorbol-12-myristate 13-acetate; activators of the cyclic AMP (cAMP) and protein kinase C (PKC) second messenger pathways, respectively. Levels of CCK mRNA were determined after 6, 12 and 24 hour drug treatments, with Northern blot analysis using human CCK cDNA hybridization probes. Activation of both cAMP and PKC second messenger pathways increased CCK mRNA levels in SK-N-MCIXC cells. These results indicate that the levels of CCK mRNA in SK-N-MCIXC cells are regulated by cAMP and PKC dependent mechanisms.

摘要

在人神经上皮瘤细胞系SK-N-MCIXC中研究了胆囊收缩素（CCK）表达的调控。分别用磷酸二酯酶抑制剂异丁基甲基黄嘌呤和促肿瘤佛波酯佛波醇-12-肉豆蔻酸酯13-乙酸酯处理细胞，它们分别是环磷酸腺苷（cAMP）和蛋白激酶C（PKC）第二信使途径的激活剂。在药物处理6、12和24小时后，使用人CCK cDNA杂交探针通过Northern印迹分析来测定CCK mRNA的水平。cAMP和PKC第二信使途径的激活均增加了SK-N-MCIXC细胞中CCK mRNA的水平。这些结果表明，SK-N-MCIXC细胞中CCK mRNA的水平受cAMP和PKC依赖性机制调控。