Ishiguro H, Hayakawa T, Kondo T, Shibata T, Kitagawa M, Sakai Y, Sobajima H, Nakae Y, Tanikawa M
2nd Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Digestion. 1993;54(4):207-12. doi: 10.1159/000201039.
To clarify whether somatostatin has an inhibitory effect on pancreatic acinar cells, we studied the effect of a long-acting analogue of somatostatin, octreotide, on amylase secretion from isolated mouse pancreatic acini. Octreotide (100 nM) had no inhibitory effects on amylase secretion stimulated by secretin or vasoactive intestinal polypeptide (VIP), while reducing the increase of cyclic adenosine 3',5'-monophosphate (cAMP). On the other hand, octreotide inhibited synergistic amylase secretion induced by secretin or VIP in combination with cholecystokinin (CCK). Octreotide also reduced synergistic amylase secretion by secretin or VIP in combination with calcium ionophore A23187. CCK and A23187 did not alter the increase of cAMP induced by secretin and the inhibitory effect of octreotide on cAMP production. Octreotide did not significantly inhibit amylase secretion stimulated by dibutyryl cAMP (dbcAMP) alone, but reduced synergistic amylase secretion by dbcAMP+A23187. Results obtained above reveal that octreotide has a direct inhibitory effect on amylase secretion from mouse pancreatic acini and probably affects stimulus secretion coupling at a point distal to the production of cAMP, besides inhibiting adenylate cyclase.
为了阐明生长抑素是否对胰腺腺泡细胞具有抑制作用，我们研究了生长抑素的长效类似物奥曲肽对分离的小鼠胰腺腺泡淀粉酶分泌的影响。奥曲肽（100 nM）对促胰液素或血管活性肠肽（VIP）刺激的淀粉酶分泌没有抑制作用，同时减少了环磷酸腺苷（cAMP）的增加。另一方面，奥曲肽抑制了促胰液素或VIP与胆囊收缩素（CCK）联合诱导的协同淀粉酶分泌。奥曲肽还减少了促胰液素或VIP与钙离子载体A23187联合诱导的协同淀粉酶分泌。CCK和A23187并未改变促胰液素诱导的cAMP增加以及奥曲肽对cAMP产生的抑制作用。奥曲肽单独对二丁酰cAMP（dbcAMP）刺激的淀粉酶分泌没有显著抑制作用，但减少了dbcAMP + A23187诱导的协同淀粉酶分泌。上述结果表明，奥曲肽对小鼠胰腺腺泡的淀粉酶分泌具有直接抑制作用，除了抑制腺苷酸环化酶外，可能还在cAMP产生的远端影响刺激-分泌偶联。
