Yotsumoto F, Manabe T, Ohshio G
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Digestion. 1993;54(4):224-30. doi: 10.1159/000201041.
This study was designed to investigate the role of bradykinin in the aggravation of acute pancreatitis. After injection of bradykinin 2 micrograms/kg to anesthetized rabbits with cerulein-induced acute pancreatitis, the pancreatic blood flow through gastroduodenal and superior mesenteric arteries (GDAF and SMAF) was determined with electromagnetic blood flow meters, the serum amylase level was measured, and pancreatic tissue was observed histologically. In rabbits treated with a supramaximal dose of cerulein alone (20 micrograms/kg/h), pancreatic blood flow was decreased and the serum amylase level was increased significantly by the early phase, and histological examination showed acute edematous pancreatitis. In rabbits treated with cerulein and bradykinin, GDAF and SMAF were significantly diminished at 300 min (51 +/- 5% and 50 +/- 4%, respectively, p < 0.05), and the serum amylase level rose significantly at 180 and 300 min (730 +/- 130% and 1,190 +/- 200%, respectively, p < 0.01) compared with rabbits treated with cerulein alone, and histological examination revealed pancreatic necrosis and greater inflammatory cell infiltration. These findings suggest that bradykinin has an additive role in the aggravation of acute pancreatitis.
本研究旨在探讨缓激肽在急性胰腺炎病情加重过程中的作用。给经蛙皮素诱导产生急性胰腺炎的麻醉兔注射2微克/千克缓激肽后，用电磁血流仪测定经胃十二指肠动脉和肠系膜上动脉的胰腺血流（GDAF和SMAF）水平，检测血清淀粉酶水平，并对胰腺组织进行组织学观察。单独用超大剂量蛙皮素（20微克/千克/小时）处理的兔，胰腺血流在早期即下降，血清淀粉酶水平显著升高，组织学检查显示为急性水肿性胰腺炎。在用蛙皮素和缓激肽处理的兔中，300分钟时GDAF和SMAF显著降低（分别为51±5%和50±4%，p<0.05），与单独用蛙皮素处理的兔相比，血清淀粉酶水平在180分钟和300分钟时显著升高（分别为730±130%和1190±200%，p<0.01），组织学检查显示胰腺坏死且炎症细胞浸润更严重。这些结果表明，缓激肽在急性胰腺炎病情加重方面具有累加作用。
