肿瘤坏死因子与豚鼠感染皮钦德病毒的发病机制
Tumor necrosis factor and the pathogenesis of Pichinde virus infection in guinea pigs.
作者信息
Aronson J F, Herzog N K, Jerrells T R
机构信息
Department of Pathology, University of Texas Medical Branch, Galveston, USA.
出版信息
Am J Trop Med Hyg. 1995 Mar;52(3):262-9. doi: 10.4269/ajtmh.52-262.
Pichinde virus (PIC) is a reticuloendothelial arenavirus of the New World tropics. A guinea pig passage-adapted strain of this virus (adPIC) is uniformly lethal for inbred guinea pigs, while the related, prototype strain (PIC3739) has attenuated virulence. The abilities of adPIC and PIC3739 to induce tumor necrosis factor (TNF) in vivo and in cultured macrophages were compared. Infection with adPIC, but not PIC3739, was associated with detectable serum TNF that peaked in week 2 of infection. Tumor necrosis factor was found in the spleens of adPIC- and PIC3739-infected animals in week 1 of infection; TNF alpha mRNA levels in spleens and livers of adPIC infected animals increased and remained high throughout infection, whereas PIC3739-infected organs showed down regulation of TNF alpha mRNA late in infection. Peritoneal macrophages explanted from adPIC-infected animals showed enhanced lipopolysaccharide-inducible TNF production. Altered regulation of TNF production may play a role in the pathogenesis of guinea pig arenavirus disease.
皮钦德病毒（PIC）是一种新世界热带地区的网状内皮系统沙粒病毒。该病毒的一种经豚鼠传代适应的毒株（adPIC）对近交系豚鼠具有一致的致死性，而相关的原型毒株（PIC3739）毒力已减弱。比较了adPIC和PIC3739在体内和培养的巨噬细胞中诱导肿瘤坏死因子（TNF）的能力。感染adPIC（而非PIC3739）与可检测到的血清TNF有关，其在感染第2周达到峰值。在感染第1周，在感染adPIC和PIC3739的动物脾脏中发现了肿瘤坏死因子；adPIC感染动物脾脏和肝脏中的TNFα mRNA水平在整个感染过程中升高并保持在较高水平，而PIC3739感染的器官在感染后期显示TNFα mRNA下调。从感染adPIC的动物中分离出的腹腔巨噬细胞显示脂多糖诱导的TNF产生增强。TNF产生的调节改变可能在豚鼠沙粒病毒病的发病机制中起作用。
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