Mascellani G, Liverani L, Prete A, Bergonzini G, Bianchini P, Torri G, Bisio A, Guerrini M, Casu B
Opocrin S.p.A. R&D Laboratorio, Corlo di Formigine, Modena, Italy.
Anal Biochem. 1994 Nov 15;223(1):135-41. doi: 10.1006/abio.1994.1558.
The sequence (IdoA2SO3-GalNAc4SO3)n contributes to the HCII-mediated inhibition of thrombin by dermatan sulfate (DS). This sequence clearly results from the 13C NMR spectrum and can be quantified by the signal C1-H of IdoA2SO3 in the 1H NMR spectrum. A linear correlation has been found between the content in the disulfated disaccharide delta Di-2,4diS obtained by enzymatic demolition with ABC lyase, the percentage content in IdoA2SO3 quantified by 1H NMR, and the HCII-mediated activity of dermatan sulfates from beef mucosa and pig skin. DSs have been obtained also from pig mucosa and contain an amount, not negligible, of delta Di-4, 6diS. This disulfate disaccharide contributes to the activity expressed by the IdoA2SO3-GalNAc4SO3 sequence. The analytical techniques HPLC and 1H NMR, applied to the currently performed analyses of DS, are described and discussed.
序列(艾杜糖醛酸2-硫酸酯- N -乙酰半乳糖胺4-硫酸酯)n有助于硫酸皮肤素(DS)介导的HCII对凝血酶的抑制作用。该序列可通过13C NMR光谱明确得出，并可通过1H NMR光谱中艾杜糖醛酸2-硫酸酯的信号C1-H进行定量。通过ABC裂合酶酶解得到的二硫酸化二糖δDi-2,4diS的含量、通过1H NMR定量的艾杜糖醛酸2-硫酸酯的百分比含量以及来自牛黏膜和猪皮肤的硫酸皮肤素的HCII介导活性之间已发现线性相关性。硫酸皮肤素也从猪黏膜中获得，并且含有一定量（不可忽略）的δDi-4,6diS。这种二硫酸化二糖对由艾杜糖醛酸2-硫酸酯- N -乙酰半乳糖胺4-硫酸酯序列所表现出的活性有贡献。描述并讨论了应用于当前硫酸皮肤素分析的分析技术HPLC和1H NMR。
