Bourinbaiar A S, Lee-Huang S
Department of Biochemistry, New York University School of Medicine, NY 10016.
Biochem Biophys Res Commun. 1995 Mar 17;208(2):779-85. doi: 10.1006/bbrc.1995.1405.
MAP30 is an antiviral protein from bitter melon (Momordica charantia). The enhancement of weak HIV antagonists, dexamethasone and indomethacin, by MAP30 has been examined by measuring the reduction in p24 expression in acutely infected MT-4 lymphocytes. In the presence of 1.5 nM MAP30 the IC50 dose of dexamethasone and indomethacin has been lowered, without concurrent cytotoxicity, at least a thousand-fold to 10(-7) M and 10(-8) M, respectively. This observation indicates that MAP30, a multifunctional antiviral plant protein capable of topological inactivation of viral DNA and specific cleavage of 28 S ribosomal RNA, may regulate HIV replication in concert with steroid and non-steroidal inhibitors of prostaglandin synthesis. The results suggest that use of MAP30 in combination with low pharmacological doses of dexamethasone and indomethacin may improve the efficacy of anti-HIV therapy.
MAP30是一种来自苦瓜（苦瓜属）的抗病毒蛋白。通过测量急性感染的MT-4淋巴细胞中p24表达的降低，研究了MAP30对弱HIV拮抗剂地塞米松和吲哚美辛的增强作用。在存在1.5 nM MAP30的情况下，地塞米松和吲哚美辛的IC50剂量降低，且无同时发生的细胞毒性，至少降低了一千倍，分别降至10(-7) M和10(-8) M。这一观察结果表明，MAP30是一种能够对病毒DNA进行拓扑失活并特异性切割28 S核糖体RNA的多功能抗病毒植物蛋白，可能与前列腺素合成的类固醇和非类固醇抑制剂协同调节HIV复制。结果表明，将MAP30与低药理剂量的地塞米松和吲哚美辛联合使用可能会提高抗HIV治疗的疗效。
