人类抑制蛋白（SAG）、β-抑制蛋白2（ARRB2）和β-肾上腺素能受体激酶2（ADRBK2）基因的染色体定位

Chromosome mapping of the human arrestin (SAG), beta-arrestin 2 (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes.

作者信息

Calabrese G, Sallese M, Stornaiuolo A, Stuppia L, Palka G, De Blasi A

机构信息

Consorzio Mario Negri Sud, Istituto di Ricerche Farmacologiche Mario Negri, Santa Maria Imbaro, Italy.

出版信息

Genomics. 1994 Sep 1;23(1):286-8. doi: 10.1006/geno.1994.1497.

DOI:10.1006/geno.1994.1497

PMID:7695743

Abstract

Two types of proteins play a major role in determining homologous desensitization of G-coupled receptors: beta-adrenergic receptor kinase (beta ARK), which phosphorylates the agonist-occupied receptor and its functional cofactor, beta-arrestin. Both beta ARK and beta-arrestin are members of multigene families. The family of G-protein-coupled receptor kinases includes rhodopsin kinase, beta ARK1, beta ARK2, IT11-A (GRK4), GRK5, and GRK6. The arrestin/beta-arrestin gene family includes arrestin (also known as S-antigen), beta-arrestin 1, and beta-arrestin 2. Here we report the chromosome mapping of the human genes for arrestin (SAG), beta-arrestin 2 (ARRB2), and beta ARK2 (ADRBK2) by fluorescence in situ hybridization (FISH). FISH results confirmed the assignment of the gene coding for arrestin (SAG) to chromosome 2 and allowed us to refine its localization to band q37. The gene coding for beta-arrestin 2 (ARRB2) was mapped to chromosome 17p13 and that coding for beta ARK2 (ADRBK2) to chromosome 22q11.

摘要

两类蛋白质在决定G蛋白偶联受体的同源脱敏中起主要作用：β-肾上腺素能受体激酶（βARK），它使激动剂占据的受体及其功能辅助因子β-抑制蛋白磷酸化。βARK和β-抑制蛋白都是多基因家族的成员。G蛋白偶联受体激酶家族包括视紫红质激酶、βARK1、βARK2、IT11-A（GRK4）、GRK5和GRK6。抑制蛋白/β-抑制蛋白基因家族包括抑制蛋白（也称为S抗原）、β-抑制蛋白1和β-抑制蛋白2。在此，我们通过荧光原位杂交（FISH）报告了人类抑制蛋白（SAG）、β-抑制蛋白2（ARRB2）和βARK2（ADRBK2）基因的染色体定位。FISH结果证实了编码抑制蛋白（SAG）的基因定位于2号染色体，并使我们能够将其定位精确到q37带。编码β-抑制蛋白2（ARRB2）的基因定位于17号染色体p13，编码βARK2（ADRBK2）的基因定位于22号染色体q11。