Kasza A, Bugno M, Koj A
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Biol Chem Hoppe Seyler. 1994 Nov;375(11):779-83. doi: 10.1515/bchm3.1994.375.11.779.
HepG2 cells were cultured for 7 days in serum-free medium in the presence of interleukin-6 (IL-6), retinoic acid (RA) or dexamethasone (DX), and some plasma proteins secreted to the media were determined by electroimmunoassay whereas the contents of specific mRNAs in the cells was evaluated by Northern blot hybridization. Interleukin-6 maximally stimulated synthesis of alpha-1-antichymotrypsin between days 1 and 3 whereas the response of fibrinogen was delayed to days 3 to 7. Retinoic acid increased the effect of IL-6 on alpha-1-antichymotrypsin (ACT) and fibrinogen (FBG) on the level of both proteins and mRNAs. Synthesis of albumin was slightly inhibited by IL-6 and RA, and synthesis of transferrin was increased by RA but not by IL-6. Dexamethasone had small enhancing effect on the action of IL-6. These results suggest that long-term HepG2 cultures may provide an experimental model for liver acute phase response during chronic inflammation.
将HepG2细胞在无血清培养基中于白细胞介素-6（IL-6）、视黄酸（RA）或地塞米松（DX）存在的情况下培养7天，通过电免疫测定法测定分泌到培养基中的一些血浆蛋白，而通过Northern印迹杂交评估细胞中特定mRNA的含量。白细胞介素-6在第1天至第3天最大程度地刺激了α-1-抗糜蛋白酶的合成，而纤维蛋白原的反应则延迟至第3天至第7天。视黄酸增强了IL-6对α-1-抗糜蛋白酶（ACT）和纤维蛋白原（FBG）在蛋白质和mRNA水平上的作用。白蛋白的合成受到IL-6和RA的轻微抑制，转铁蛋白的合成受到RA的增加但不受IL-6的影响。地塞米松对IL-6的作用有较小的增强作用。这些结果表明，长期的HepG2培养可能为慢性炎症期间肝脏急性期反应提供一个实验模型。
