Sugimoto T, Maruo K, Imaeda Y, Suzuki K, Shirota K, Ejima H, Endo S, Nomura T
Department of Veterinary Surgery, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
J Vet Med Sci. 1994 Dec;56(6):1087-91. doi: 10.1292/jvms.56.1087.
Canine tumors resected at surgery or autopsy were subcutaneously xenotransplanted into SCID mice. Thirty of the seventy-three tumors (41.1%) grew primarily in SCID mice. The primary take rate of mammary tumors and skin and subcutaneous tumors was 55.9% (19/34) and 28.6% (8/28), respectively. One thyroid carcinoma, one oral leiomyosarcoma and one osteosarcoma also underwent successful primary growth. Thirteen of the forty-one benign tumors (31.7%) and 17 of the thirty-two malignant ones (53.1%) were successfully transplantable. The histological features of the primary xenografts and their original tumors were similar to each other in all the cases. Three xenografts of the four malignant tumors with metastasis in the canine patients were shown to grow also in SCID mice and two mammary tumor xenografts out of the three metastasized to the lungs of SCID mice as well. All the 30 primary tumor xenografts grown in the first recipient were successfully transplantable to the second generation of the SCID mice. Xenotransplantation using SCID mice appears to be a valuable tool for investigating the biological characteristics of canine neoplasmas.
将在手术或尸检时切除的犬类肿瘤皮下异种移植到SCID小鼠体内。73个肿瘤中有30个（41.1%）在SCID小鼠体内主要生长。乳腺肿瘤以及皮肤和皮下肿瘤的初次接种成功率分别为55.9%（19/34）和28.6%（8/28）。1例甲状腺癌、1例口腔平滑肌肉瘤和1例骨肉瘤也实现了初次成功生长。41个良性肿瘤中有13个（31.7%）、32个恶性肿瘤中有17个（53.1%）可成功移植。在所有病例中，初次异种移植瘤与其原发肿瘤的组织学特征相似。犬类患者中4个发生转移的恶性肿瘤的3个异种移植瘤在SCID小鼠中也能生长，3个乳腺肿瘤异种移植瘤中有2个也转移至SCID小鼠的肺部。在第一代受体中生长的所有30个原发肿瘤异种移植瘤均可成功移植到第二代SCID小鼠体内。利用SCID小鼠进行异种移植似乎是研究犬类肿瘤生物学特性的一种有价值的工具。
