动物胎盘胱氨酸氨基肽酶的洗脱曲线和分子量

Elution profiles and molecular weights of placental cystine aminopeptidase in animals.

作者信息

Ikenaga H, Ono K, Tomoda I

机构信息

Department of Veterinary Clinical Pathology, School of Veterinary Medicine, Nippon Veterinary and Animal Science University, Tokyo, Japan.

出版信息

J Vet Med Sci. 1994 Dec;56(6):1139-42. doi: 10.1292/jvms.56.1139.

DOI:10.1292/jvms.56.1139

PMID:7696406

Abstract

Gel filtration was performed for cystine aminopeptidase (CAP) [EC 3.4.11.3] on full term placental extracts from human, cynomolgus monkey, dog, goat, pig and horse. The enzymatic profiles of CAP were examined and compared with that of CAP in pregnancy plasma on the basis of inhibitory effects. Elution profiles of placental extracts exhibited 2 CAP activity peaks in human and pig, 3 peaks in cynomolgus monkey, dog and goat, and 4 peaks in horse. The molecular weights of placental CAP that showed identical inhibitory effects to that of pregnancy plasma CAP were estimated to be approximately 325,000 in human, 350,000 in the cynomolgus monkey, 140,000 in the dog, 140,000 in the goat, 128,000 in the pig, and 115,000 in the horse. These molecular weights tended to decrease in accordance with the increase of barrier layers present between maternal blood and placental syncytiotrophoblasts in which CAP is synthesized.

摘要

对人、食蟹猴、狗、山羊、猪和马的足月胎盘提取物进行了胱氨酸氨基肽酶（CAP）[EC 3.4.11.3]的凝胶过滤。根据抑制作用，检查了CAP的酶谱并与妊娠血浆中的CAP酶谱进行了比较。胎盘提取物的洗脱图谱显示，人胎盘和猪胎盘提取物中有2个CAP活性峰，食蟹猴、狗和山羊胎盘提取物中有3个活性峰，马胎盘提取物中有4个活性峰。与妊娠血浆CAP表现出相同抑制作用的胎盘CAP的分子量估计在人中约为325,000，在食蟹猴中为350,000，在狗中为140,000，在山羊中为140,000，在猪中为128,000，在马中为115,000。这些分子量倾向于随着母体血液与合成CAP的胎盘合体滋养层之间存在的屏障层的增加而降低。