Ishihara C, Tsuji M, Hagiwara K, Hioki K, Arikawa J, Azuma I
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
J Vet Med Sci. 1994 Dec;56(6):1149-54. doi: 10.1292/jvms.56.1149.
We have previously demonstrated that the red blood cells (RBCs) in the blood circulation of SCID mice could be almost completely substituted with bovine RBCs by means of repeated transfusions. In the present study, transfusion experiments were carried out with RBCs from various animal species to investigate the ability of SCID mice to accept xenogeneic RBCs. Bovine (Bo), equine (Eq), human (Hu) and murine (Mu) RBCs were labeled with a fluorescent dye to trace their trafficking in the blood stream. Following the intravenous injection these RBCs were cleared from the circulation at various rates; the 1/100 reduction time in the labeled cell counts was 2 to 7 days, 4 to 7 hr and 1 to 2 hr with Bo-, Hu- and Eq-RBCs, respectively. In contrast, labeled Mu-RBCs from not only syngeneic but also allogeneic mouse strain were able to stay over 50 days. The difference in clearance rates was attributable to the difference in uptake of the RBCs by the splenic macrophages. The clearance rates of labeled RBCs were significantly decreased by co-transfusion with unlabeled RBCs of the same species, indicating that xenogeneic RBCs were recognized by the reticulo-endothelial system of SCID mice in a species-specific fashion. Furthermore, at least in the case of Hu-RBC, the complement component 3 may play a role in the Hu-RBC clearance in SCID mice since C3 deposition was observed on Hu-RBCs but not on Bo- and Eq-RBCs.
我们之前已经证明，通过反复输血，SCID小鼠血液循环中的红细胞（RBCs）几乎可以完全被牛红细胞替代。在本研究中，用来自不同动物物种的红细胞进行输血实验，以研究SCID小鼠接受异种红细胞的能力。牛（Bo）、马（Eq）、人（Hu）和鼠（Mu）的红细胞用荧光染料标记，以追踪它们在血流中的运行情况。静脉注射后，这些红细胞以不同速率从循环中清除；标记细胞计数减少1/100的时间，牛红细胞、人红细胞和马红细胞分别为2至7天、4至7小时和1至2小时。相比之下，来自同基因以及同种异体小鼠品系的标记鼠红细胞能够留存超过50天。清除率的差异归因于脾巨噬细胞对红细胞摄取的差异。与相同物种的未标记红细胞共同输血可显著降低标记红细胞的清除率，这表明异种红细胞以物种特异性方式被SCID小鼠的网状内皮系统识别。此外，至少在人红细胞的情况下，补体成分3可能在SCID小鼠清除人红细胞的过程中发挥作用，因为在人红细胞上观察到了C3沉积，而在牛红细胞和马红细胞上未观察到。
