Sogo M T, Gigirey M D, Tutor J C
Laboratorio Central, Hospital General de Galicia, Clínico Universitario, Santiago de Compostela, Spain.
Eur J Clin Chem Clin Biochem. 1994 Dec;32(12):905-8.
Using adenosine 5'-monophosphate (Arkesteijn; J. Clin. Chem. Clin. Biochem. 14 (1976) 155) and inosine 5'-monophosphate (Bertrand & Buret; Clin. Chim. Acta 119 (1982) 275) as substrates for the determination of serum 5'-nucleotidase, linear Arrhenius plots were obtained between 25 degrees C and 37 degrees C. With the latter method, in patients with hepatobiliary disease, activation energy is higher than in healthy subjects (67.8 +/- 0.8 kJ.mol-1 vs. 59.2 +/- 1.6 kJ.mol-1, p < 0.001). Neuraminidase treatment resulted in a significant reduction in the activation energy of serum 5'-nucleotidase from patients (63.6 +/- 0.6 kJ.mol-1, p < 0.001). Although the activation energy seen is also influenced by conditions other than sialic acid content, the results obtained are in accordance with the hypothesis that in liver disease serum 5'-nucleotidase becomes more sialylated.
使用5'-单磷酸腺苷（阿克斯特因；《临床化学与临床生物化学杂志》14 (1976) 155）和5'-单磷酸肌苷（伯特兰和比雷；《临床化学学报》119 (1982) 275）作为测定血清5'-核苷酸酶的底物，在25℃至37℃之间获得了线性阿伦尼乌斯图。采用后一种方法，在肝胆疾病患者中，活化能高于健康受试者（67.8±0.8 kJ·mol-1对59.2±1.6 kJ·mol-1，p<0.001）。神经氨酸酶处理导致患者血清5'-核苷酸酶的活化能显著降低（63.6±0.6 kJ·mol-1，p<0.001）。尽管所观察到的活化能也受唾液酸含量以外的其他条件影响，但所得结果符合以下假设：在肝脏疾病中，血清5'-核苷酸酶的唾液酸化程度更高。
