Tóth-Boconádi R, Taneva S G, Keszthelyi L
Institute of Biophysics, Biological Research Center, Szeged, Hungary.
Biophys J. 1994 Dec;67(6):2490-2. doi: 10.1016/S0006-3495(94)80737-4.
Large conductivity changes have been measured during the photocycle of bacteriorhodopsin in purple membrane. These phenomena were explained as being due to the occurrence of large-scale non-proton ion release. Here we show that these conductivity changes do not appear if the purple membrane is immobilized. We propose an alternative hypothesis that explains the presence of conductivity change in suspensions and their absence in gels, as well as several related effects suggesting that the observed conductivity changes are due to alteration of the polarizability of purple membrane during the photocycle.
在紫色膜中细菌视紫红质的光循环过程中，已测量到大幅度的电导率变化。这些现象被解释为大规模非质子离子释放的结果。在此我们表明，如果紫色膜被固定，这些电导率变化就不会出现。我们提出了另一种假说，该假说解释了悬浮液中电导率变化的存在及其在凝胶中不存在的原因，以及一些相关效应，表明观察到的电导率变化是由于光循环过程中紫色膜极化率的改变所致。
