紫膜中的非质子离子释放
Non-proton ion release in purple membrane.
作者信息
Tóth-Boconádi R, Taneva S G, Keszthelyi L
机构信息
Institute of Biophysics, Biological Research Center, Szeged, Hungary.
出版信息
Biophys J. 1994 Dec;67(6):2490-2. doi: 10.1016/S0006-3495(94)80737-4.
Abstract
Large conductivity changes have been measured during the photocycle of bacteriorhodopsin in purple membrane. These phenomena were explained as being due to the occurrence of large-scale non-proton ion release. Here we show that these conductivity changes do not appear if the purple membrane is immobilized. We propose an alternative hypothesis that explains the presence of conductivity change in suspensions and their absence in gels, as well as several related effects suggesting that the observed conductivity changes are due to alteration of the polarizability of purple membrane during the photocycle.
摘要
在紫色膜中细菌视紫红质的光循环过程中，已测量到大幅度的电导率变化。这些现象被解释为大规模非质子离子释放的结果。在此我们表明，如果紫色膜被固定，这些电导率变化就不会出现。我们提出了另一种假说，该假说解释了悬浮液中电导率变化的存在及其在凝胶中不存在的原因，以及一些相关效应，表明观察到的电导率变化是由于光循环过程中紫色膜极化率的改变所致。
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本文引用的文献
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Quantum efficiency of the photochemical cycle of bacteriorhodopsin.菌紫质光化学循环的量子效率。
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Restriction of motion of protein side chains during the photocycle of bacteriorhodopsin.细菌视紫红质光循环过程中蛋白质侧链的运动受限。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7273-7. doi: 10.1073/pnas.79.23.7273.
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Electro-optical measurements on aqueous suspension of purple membrane from Halobacterium halobium.嗜盐菌紫色膜水悬浮液的电光测量
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Absolute quantum yields and proof of proton and nonproton transient release and uptake in photoexcited bacteriorhodopsin.光激发细菌视紫红质中的绝对量子产率以及质子和非质子瞬态释放与摄取的证据。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):178-80. doi: 10.1073/pnas.80.1.178.
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Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.嗜盐菌细胞膜的分离及其分成红色膜和紫色膜的分级分离。
Methods Enzymol. 1974;31:667-78. doi: 10.1016/0076-6879(74)31072-5.
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Time-resolved photoelectric and absorption signals from oriented purple membranes immobilized in gel.来自固定在凝胶中的定向紫膜的时间分辨光电信号和吸收信号。
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Large scale nonproton ion release and bacteriorhodopsin's state of aggregation in lipid vesicles. I. Monomers.脂质体中大规模非质子离子释放及细菌视紫红质的聚集状态。I. 单体
Biophys J. 1987 Jul;52(1):115-21. doi: 10.1016/S0006-3495(87)83195-8.
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Biophys J. 1987 Jun;51(6):875-81. doi: 10.1016/S0006-3495(87)83415-X.
10
Nonproton ion release by purple membranes exhibits cooperativity as shown by determination of the optical cross-section.紫色膜的非质子离子释放表现出协同性，如通过光学截面测定所示。
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