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嗜盐菌紫色膜水悬浮液的电光测量

Electro-optical measurements on aqueous suspension of purple membrane from Halobacterium halobium.

作者信息

Barabás K, Dér A, Dancsházy Z, Ormos P, Keszthelyi L, Marden M

出版信息

Biophys J. 1983 Jul;43(1):5-11. doi: 10.1016/S0006-3495(83)84317-3.

Abstract

The permanent dipole moment, polarizability, and the retinal angle of Halobacterium halobium purple membranes were determined at different pH values. All of the parameters have a maximum between pH 5 and 6. There is a reversal in the direction of the permanent dipole moment near pH 5. The value of permanent dipole moment was determined to be 60 D/protein at pH 6.6, and the value obtained for polarizability was 3 X 10(-28) Fm2/membrane fragment. The retinal angle of all-trans retinal was 0.8 degrees smaller than that of the 13-cis conformation.

摘要

在不同pH值下测定了嗜盐菌紫膜的永久偶极矩、极化率和视黄醛角。所有参数在pH 5至6之间出现最大值。在pH 5附近,永久偶极矩的方向发生反转。在pH 6.6时,永久偶极矩的值确定为60 D/蛋白质,极化率的值为3×10(-28) Fm2/膜片段。全反式视黄醛的视黄醛角比13-顺式构象的视黄醛角小0.8度。

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