电位滴定过程中细胞色素aa3的近红外光谱变化
Near infrared spectral changes of cytochrome aa3 during potentiometric titrations.
作者信息
Hendler R W, Harmon P A, Levin I W
机构信息
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
Biophys J. 1994 Dec;67(6):2493-500. doi: 10.1016/S0006-3495(94)80739-8.
Abstract
Singular value decomposition (SVD) was used to deconvolute the spectral changes occurring in the near infrared region during potentiometric titrations of cytochrome aa3. Overall oxidized minus reduced difference spectra revealed a broad absorbance feature centered near 830 nm with an apparent Em near 250 mV. However, SVD did not isolate any spectral species with an absorbance centered near 830 nm. It was found that the spectral changes occurring in the wavelength region from 650 to 950 nm were associated mainly with cytochromes a and a3. It was concluded that the absorbance at 830 nm should not be used as an independent measure of the concentration of CuA in cytochrome aa3.
摘要
奇异值分解（SVD）用于对细胞色素aa3电位滴定过程中近红外区域发生的光谱变化进行去卷积。总的氧化态减去还原态差异光谱显示，在830 nm附近有一个宽吸收峰，表观电位（Em）接近250 mV。然而，奇异值分解并没有分离出在830 nm附近有吸收峰的任何光谱物种。研究发现，650至950 nm波长区域内发生的光谱变化主要与细胞色素a和a3有关。得出的结论是，830 nm处的吸光度不应作为细胞色素aa3中铜A浓度的独立测量指标。
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